Glycosylation status of serum in inflammatory arthritis in response to anti-TNF treatment
Glycosylation status of serum in inflammatory arthritis in response to anti-TNF treatment
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DOI：
10.1093/rheumatology/ket189
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发表时间：
2013-09-01
期刊：
影响因子：
5.5
通讯作者：
FitzGerald, Oliver
中科院分区：
文献类型：
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作者：
Collins, Emily S.;Galligan, Marie C.;FitzGerald, Oliver
方法：研究方法。54例IA患者于治疗前、抗肿瘤坏死因子治疗后1个月和12个月采集血清标本。采用基于高通量亲水相互作用的高效液相色谱方法对全血清样品中的N-糖链进行分析。抗肿瘤坏死因子治疗后，IA患者血清蛋白糖基化水平发生明显变化。我们观察到来自免疫球蛋白的半乳糖化多糖增加，核心岩藻糖化的双天线半乳糖糖基化的糖链增加，含和不含外臂岩藻糖的唾液酸化的三天线糖链减少。这种半乳糖化免疫球蛋白糖链的增加表明N-糖链的逆转，使其趋于正常的健康状态。这些变化与C反应蛋白的降低密切相关，提示糖化改变与炎症过程的减少之间存在联系。抗肿瘤坏死因子治疗后IA患者血清糖化水平的变化与炎症过程的减少密切相关，反映了抗肿瘤坏死因子对免疫系统的影响。
Methods. Serum samples were collected from 54 IA patients before treatment and at 1 and 12 months after commencing anti-TNF therapy. N-linked glycans from whole serum samples were analysed using a high-throughput hydrophilic interaction liquid chromatography-based method.Results. Glycosylation on the serum proteins of IA patients changed significantly with anti-TNF treatment. We observed an increase in galactosylated glycans from IgG, also an increase in core-fucosylated biantennary galactosylated glycans and a decrease in sialylated triantennary glycans with and without outer arm fucose. This increase in galactosylated IgG glycans suggests a reversing of the N-glycome towards normal healthy profiles. These changes are strongly correlated with decreasing CRP, suggesting a link between glycosylation changes and decreases in inflammatory processes.Conclusion. Glycosylation changes in the serum of IA patients on anti-TNF therapy are strongly associated with a decrease in inflammatory processes and reflect the effect of anti-TNF on the immune system.