Protein Imaging of CCN2 and CCN3 in Living Cells.

Protein Imaging of CCN2 and CCN3 in Living Cells.
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活细胞中 CCN2 和 CCN3 的蛋白质成像。

DOI:
10.1007/978-1-4939-6430-7_20
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发表时间:
2016
期刊:
Methods Mol Biol.
影响因子:
--
通讯作者:
and Takigawa M
and Takigawa M
中科院分区:
--
文献类型:
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作者:
Hattori T;Hoshijima M;and Takigawa M

文献摘要

相似文献

分子成像技术的最新进展对提高我们对活细胞中分子易位、受体内化和相互作用的理解产生了重大影响。本章的方案介绍了一种在活细胞中对CCN2和CCN3进行细胞内定位的优化技术,一种是使用gfp标记的CCN2和halo标记的CCN3。
Recent progress in molecular imaging technology has had a strong impact on improving our understanding of molecular translocation, receptor internalization, and interactions in living cells. The protocol in this chapter introduces an optimized technique for intracellular localization of CCN2 and CCN3 in live cells, one using GFP-tagged CCN2 and Halo-tagged CCN3.