Complementation of the leuB6 allele of Escherichia coli by cloned DNA from Mucor racemosus.
Complementation of the leuB6 allele of Escherichia coli by cloned DNA from Mucor racemosus.
复制标题
通过从总状毛霉克隆 DNA 来补充大肠杆菌的 leuB6 等位基因。
DOI：
10.1128/jb.151.1.521-523.1982
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发表时间：
1982
影响因子：
3.2
通讯作者：
Sypherd,PS
中科院分区：
文献类型：
--
作者：
Cihlar,RL;Sypherd,PS
由总状毛霉基因组 DNA 和大肠杆菌质粒 pBR322 构建重组质粒，命名为 pMu leu1。该质粒显然是通过抑制来补充大肠杆菌的 leuB6 突变。该质粒含有两个大约 3.0 和 1.7 KB 的 HindIII 片段。没有一个片段单独表现出互补活性。质粒指定了几种蛋白质，但它们在互补中的作用（如果有的话）尚不清楚。
A recombinant plasmid, designated pMu leu1, was constructed from Mucor racemosus genomic DNA and Escherichia coli plasmid pBR322. This plasmid complemented the leuB6 mutation of E. coli, apparently by suppression. The plasmid contained two HindIII fragments of approximately 3.0 and 1.7 kilobases. Neither fragment alone exhibited complementing activity. Several proteins were specified by the plasmid, but their role, if any, in complementation is unknown.