Identification of Parental Chromosomes in Sexual Intergeneric Hybrid Progenies between Citrus Cultivar ‘Nanpu’ Tangor and Citropsis schweinfurthii in the Subfamily Aurantioideae
Identification of Parental Chromosomes in Sexual Intergeneric Hybrid Progenies between Citrus Cultivar ‘Nanpu’ Tangor and Citropsis schweinfurthii in the Subfamily Aurantioideae
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DOI：
10.2503/jjshs1.79.129
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发表时间：
2010
影响因子：
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通讯作者：
K. Yasuda;M. Yahata;M. Shigyo;R. Matsumoto;T. Yabuya;H. Kunitake
中科院分区：
文献类型：
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作者：
K. Yasuda;M. Yahata;M. Shigyo;R. Matsumoto;T. Yabuya;H. Kunitake
为获得施魏氏枸橼与南浦蜜桔有性属间杂种后代的细胞遗传学信息，我们进行了色霉素A3（CMA）染色体显带和基因组原位杂交（GISH）分析。本文报道了C.施魏因富尔氏种的染色体类型非常简单（14 D +4 E），而南浦橘橙的染色体类型则较为复杂（1A + 4C + 5D +8 E）。与C.母本为schweinfurthii，核型为1A + 1C +11 D + 5E，表明A型和C型染色体均遗传自'南浦'橘鱼。GISH-CMA分析表明，杂种后代是由来自中国对虾的CMA核型为7 D +2 E的雌配子受精而产生的。schweinfurthii和南浦蜜柑的1A + 1C + 4D + 3E雄配子。结果表明，C.与野生种相比，schweinfurthii具有同形同源染色体，而'南浦' Tangor具有异形同源染色体。
To obtain cytogenetic information on sexual intergeneric hybrid progenies between Citropsis schweinfurthii and ‘Nanpu’ tangor (Citrus spp.), we conducted Chromomycin A3 (CMA) chromosome banding and genomic in situ hybridization (GISH) analyses. The CMA karyotype composition of C. schweinfurthii was very simple (14D + 4E), while that of ‘Nanpu’ tangor showed more complicated chromosome types (1A + 4C + 5D + 8E). Two progenies with C. schweinfurthii as the maternal parent showed a karyotype of 1A + 1C + 11D + 5E, suggesting that each of the type A and C chromosomes was inherited from ‘Nanpu’ tangor. GISH-CMA analysis in one of these progenies showed a clear distinction between parental chromosomes, and revealed that the hybrid progeny arose due to fertilization between a female gamete with a CMA karyotype composition of 7D + 2E from C. schweinfurthii and a male 1A + 1C + 4D + 3E gamete from ‘Nanpu’ tangor. The result suggests that C. schweinfurthii had homomorphic homologous chromosomes as a wild species, while ‘Nanpu’ tangor had heteromorphic homologous chromosomes.