Diversity of the Arabidopsis Mitochondrial Genome Occurs via Nuclear-Controlled Recombination Activity
Diversity of the Arabidopsis Mitochondrial Genome Occurs via Nuclear-Controlled Recombination Activity
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DOI：
10.1534/genetics.109.108514
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发表时间：
2009-12-01
期刊：
影响因子：
3.3
通讯作者：
Mackenzie, Sally A.
中科院分区：
文献类型：
--
作者：
Arrieta-Montiel, Maria P.;Shedge, Vikas;Mackenzie, Sally A.
植物线粒体基因组是重组发生的，DNA交换活性在很大程度上受核基因产物控制。一个核基因，MSH 1，似乎参与抑制重组在拟南芥。每个重复序列的大小在108到556 bp之间。存在于广泛的植物物种中，这些线粒体重复序列显示拟南芥中成功的不对称DNA交换的证据Adien MSH 1被破坏。重复序列的同源性和大小似乎会影响重复频率，较大的重复序列对应于增加的DNA交换活性。广泛的线粒体基因组重组的msh 1突变产生改变线粒体转录模式。拟南芥生态型C24，Col-0，和Ler的线粒体基因组的比较表明，MSH 1活性占大多数或所有的多态性区分这些基因组，产生生态型特定的化学计量的变化，在每个线。我们的观察结果表明，MSH 1参与线粒体基因组进化的影响，在高等植物线粒体遗传变异的谱系特异性模式。
The plant mitochondrial genome is recombinogenic, with DNA exchange activity controlled to a large extent by nuclear gene products. One nuclear gene, MSH1, appears to participate in suppressing recombination in Arabidopsis at. every repeated sequence ranging in size front 108 to 556 bp. Present in a wide range of plant species, these mitochondrial repeats display evidence of successful asymmetric DNA exchange in Arabidopsis Adien MSH1 is disrupted. Recombination frequency appears to be influenced by repeat sequence homology and size, With larger size repeats corresponding to increased DNA exchange activity. The extensive mitochondrial genomic reorganization of the msh1 mutant produced altered mitochondrial transcription patterns. Comparison of mitochondrial genomes from the Arabidopsis ecotypes C24, Col-0, and Ler suggests that MSH1 activity accounts for most or all of the polymorphisms distinguishing these genomes, producing ecotype-specific stoichiometric changes in each line. Our observations suggest that MSH1 participates in mitochondrial genome evolution by influencing the lineage-specific pattern of mitochondrial genetic variation in higher plants.