Characterization of Rolled and Erect Leaf 1 in regulating leave morphology in rice.
Characterization of Rolled and Erect Leaf 1 in regulating leave morphology in rice.
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DOI：
10.1093/jxb/erv319
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发表时间：
2015-09
影响因子：
6.9
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Chen Q;Xie Q;Gao J;Wang W;Sun B;Liu B;Zhu H;Peng H;Zhao H;Liu C;Wang J;Zhang J;Zhang G;Zhang Z
Rolled and Erect Leaf 1（Rolled and Erect Leaf 1）基因是一个控制水稻油菜素类固醇信号传导相关叶片形态建成和叶片角度的新基因。叶片形态是作物最重要的农艺性状之一，因为它通过控制光合能力和蒸腾作用而影响产量。为了研究水稻叶片形态发生的调控机制，对水稻卷叶直立叶1（rel1）的显性突变体进行了研究。该突变体具有主要的卷叶、增加的叶角和降低的株高表型，其导致谷物产量降低。电镜观察表明，rel1显性突变体的叶片弯曲是由于泡状细胞大小和数量的改变所致。分子克隆表明，rel1显性突变表型是由激活的re11基因，它编码一种新的未知蛋白，尽管它的高度保守的单子叶植物。此外，在rel1显性突变体中，repl1基因的下调恢复了该显性突变体的表型。另外，在野生型植物中过表达的rell 1诱导的表型类似的显性rel1突变体，表明rell 1在叶片卷曲和弯曲中起着积极的作用。与所观察到的rel1表型一致，在植物生长发育过程中，REL1基因主要在各种组织的分生组织中表达。然而，两个rel1显性突变体和repl1过表达植物外源油菜素类固醇（BR）的反应性降低。此外，BR反应基因的转录水平在rel1显性突变体和repl1过表达线显着改变。此外，还从酵母双杂交筛选中鉴定了7个与BMP1相互作用的蛋白质。两者合计，这些发现表明，EST1调节叶形态，特别是在叶片卷曲和弯曲，通过BR信号转导的协调。
The Rolled and Erect Leaf 1 (REL1) gene is a novel component controlling brassinosteroid signalling-associated leaf morphogenesis and leaf angle in Oryza sativa. Leaf morphology, particularly in crop, is one of the most important agronomic traits because it influences the yield through the manipulation of photosynthetic capacity and transpiration. To understand the regulatory mechanism of leaf morphogenesis, an Oryza sativa dominant mutant, rolled and erect leaf 1 (rel1) has been characterized. This mutant has a predominant rolled leaf, increased leaf angle, and reduced plant height phenotype that results in a reduction in grain yield. Electron microscope observations indicated that the leaf incurvations of rel1 dominant mutants result from the alteration of the size and number of bulliform cells. Molecular cloning revealed that the rel1 dominant mutant phenotype is caused by the activation of the REL1 gene, which encodes a novel unknown protein, despite its high degree of conservation among monocot plants. Moreover, the downregulation of the REL1 gene in the rel1 dominant mutant restored the phenotype of this dominant mutant. Alternatively, overexpression of REL1 in wild-type plants induced a phenotype similar to that of the dominant rel1 mutant, indicating that REL1 plays a positive role in leaf rolling and bending. Consistent with the observed rel1 phenotype, the REL1 gene was predominantly expressed in the meristem of various tissues during plant growth and development. Nevertheless, the responsiveness of both rel1 dominant mutants and REL1-overexpressing plants to exogenous brassinosteroid (BR) was reduced. Moreover, transcript levels of BR response genes in the rel1 dominant mutants and REL1-overexpressing lines were significantly altered. Additionally, seven REL1-interacting proteins were also identified from a yeast two-hybrid screen. Taken together, these findings suggest that REL1 regulates leaf morphology, particularly in leaf rolling and bending, through the coordination of BR signalling transduction.