DNA Degradation of Genetically Modified Cottonseed Meal During Feed Processing
DNA Degradation of Genetically Modified Cottonseed Meal During Feed Processing
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饲料加工过程中转基因棉粕的 DNA 降解
DOI：
10.1007/s12010-012-9982-3
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发表时间：
2013-01-01
影响因子：
3
通讯作者：
Wang, Jianhua
中科院分区：
文献类型：
--
作者：
Guan, Qingfeng;Wang, Xiumin;Wang, Jianhua
采用聚合酶链反应（PCR）和实时荧光定量PCR方法研究了干热、湿热和挤压对棉籽粕（CSM）中DNA降解的影响。在所有干热 CSM 和棉籽中均可检测到大小范围为 128 至 883 bp 的 thesad1DNA 和大小范围为 183 至 652 bp 的 cry1Ab/c 基因。在湿加热过程中，thesad1 基因（≥883 bp）和 thecry1Ab/c（≥952 bp）基因分别在 105 和 120 °C 下被彻底降解。在 75 至 135 °C 温度范围的挤压过程中，检测到 thesad1 基因的大小为 128 至 530 bp，以及 thecry1Ab/c 基因的大小为 183 至 652 bp。在含水量介于 26% 和 34% 之间的所有挤压样品中均检测到 thesad1 基因的片段≤883 bp 和 thecry1Ab/c 基因的片段≤952 bp。 Bt棉籽粕样品中cry1Ab/ctosad1拷贝数比在加热过程中随温度升高而迅速下降。总之，饲料加工显着降解了sad1和cry1Ab/c的较大DNA片段，高温和含水量是降解的主要因素。
The effects of dry heating, wet heating, and extrusion on the degradation of DNA in cottonseed meal (CSM) were studied using polymerase chain reaction (PCR) and real-time PCR approach. Both thesad1DNA, ranging between 128 and 883 bp in size, and thecry1Ab/cgene, ranging between 183 and 652 bp in size, were detectable in all dry-heated CSM and cottonseed. During wet heating, thesad1gene (≥883 bp) and thecry1Ab/c(≥952 bp) gene were thoroughly degraded at 105 and 120 °C, respectively. Sizes from 128 to 530 bp for thesad1gene and sizes from 183 to 652 bp for thecry1Ab/cgene were detected during extrusion at temperatures ranging from 75 to 135 °C. Fragments ≤883 bp for thesad1gene and ≤952 bp for thecry1Ab/cgene were detected in all of the extruded samples with water content varying between 26 and 34 %. The copy number ratio ofcry1Ab/ctosad1in samples of Bt cottonseed meal decreased rapidly when the temperature increased during the heating process. In conclusion, feed processing markedly degrades the larger DNA fragments ofsad1andcry1Ab/c, with high temperature and water content being the main factors for that degradation.