Trehalase in Harmonia axyridis (Coleoptera: Coccinellidae): effects on beetle locomotory activity and the correlation with trehalose metabolism under starvation conditions
Trehalase in Harmonia axyridis (Coleoptera: Coccinellidae): effects on beetle locomotory activity and the correlation with trehalose metabolism under starvation conditions
复制标题
异色瓢虫（鞘翅目：瓢虫科）中的海藻糖酶：对饥饿条件下甲虫运动活性的影响及其与海藻糖代谢的相关性
DOI：
10.1007/s13355-014-0244-4
复制
发表时间：
2014-05-01
影响因子：
1.3
通讯作者：
Zhang, Fan
中科院分区：
文献类型：
--
作者：
Tang, Bin;Qin, Zi;Zhang, Fan
海藻糖在昆虫的能量代谢中很重要，并能保护它们免受极端环境条件的影响。为了研究海藻糖的合成和降解（海藻糖被海藻糖酶水解为葡萄糖），克隆了异色瓢虫（Harmonia axyridis (Pallas)，鞘翅目：瓢虫科）的两个可溶性海藻糖酶cDNA（HaTreh1 - 1和HaTreh1 - 2）。HaTreh mRNA在幼虫阶段的表达水平高于成虫阶段。然后研究了饥饿条件下该瓢虫的运动行为以及HaTreh1 - 1和HaTreh1 - 2的mRNA表达情况。与对照（0小时）成虫相比，饥饿8小时的异色瓢虫成虫的冲刺速度和最大移动距离增加，停顿频率降低。相反，在饥饿8到18小时的成虫中，两种HaTreh的mRNA表达迅速增加，特别是饥饿18小时的成虫中HaTreh1 - 1的表达量是对照的289倍。这些结果表明，储存的食物储备能够为异色瓢虫成虫提供维持8小时生命活动的能量。然而，当饥饿超过8小时时，瓢虫需要海藻糖和其他能量来源来提供能量以寻找食物和维持生命活动。
Trehalose is important in the energy metabolism of insects and protects them from extreme environmental conditions. To study the synthesis and degradation of trehalose, which is hydrolyzed to glucose by trehalase, two soluble trehalase cDNAs of the beetle Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) (HaTreh1-1 and HaTreh1-2) were cloned. The HaTreh mRNA expression levels were higher during the larval than adult stages. The locomotory behavior of the beetle and its mRNA expression of HaTreh1-1 and HaTreh1-2 under starvation were then investigated. Sprint speed and maximum moving distance were increased, and the pause frequency decreased in H. axyridis adults starved for 8 h compared with control (0 h) adults. In contrast, the mRNA expression of both HaTrehs increased quickly in adults starved from 8 to 18 h, with HaTreh1-1 in particular being expressed 289-fold in adults starved for 18 h versus control. These results show that stored food reserves can provide energy to sustain vital activities for 8 h in H. axyridis adults. However, the beetles require trehalose and other energy sources to provide energy to find food and for vital activities when starved for more than 8 h.