Oncogenic microRNA-142-3p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma
Oncogenic microRNA-142-3p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma
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DOI：
10.3892/ol.2015.4021
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发表时间：
2016-02-01
期刊：
影响因子：
2.9
通讯作者：
Lai, Yongqing
中科院分区：
文献类型：
--
作者：
Li, Yifan;Chen, Duqun;Lai, Yongqing
microRNAs（miRNAs/miRs）在调节致癌途径中发挥重要作用。RCC是发生在成年人中最常见的肾癌。miRNAs由于与RCC肿瘤发生相关，可作为早期检测和进展监测的生物标志物，也是分子治疗的潜在靶点而受到越来越多的关注。miRNA-142- 3 p在肾癌组织中的表达上调已被微阵列检测到，但其在肾癌组织中的表达和功能尚未得到证实。在本研究中，采用定量聚合酶链反应（PCR）技术对53例配对RCC和癌旁正常组织中miR-142- 3 p的相对表达进行定量。此外，进行伤口愈合、3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物和流式细胞术测定以分析miR-142- 3 p对细胞迁移、增殖和凋亡的影响。结果表明，miR-142- 3 p在肾细胞癌组织中的表达显著高于癌旁正常组织。化学合成的miR-142 - 3 p抑制剂诱导的miR-142 - 3 p下调显著抑制786-O和ACHN细胞的细胞迁移和增殖，并促进细胞凋亡，支持miR-142- 3 p可能在RCC中作为癌基因发挥作用的理论。miR-142- 3 p作为生物标志物和治疗靶点的潜在临床意义为进一步研究miR-142- 3 p介导的分子途径及其如何与RCC发展相关提供了理论基础。
MicroRNAs (miRNAs/miRs) serve an important role in the regulation of carcinogenic pathways. RCC is the most prevalent kidney cancer that occurs in adults. miRNAs have gained increasing attention due to their association with RCC tumorigenesis, serving as biomarkers for early detection and progression monitoring, and as potential targets for molecular therapy. Upregulation of miRNA-142-3p has been previously identified in RCC tissues by microarray profile, however, its expression and function in RCC have not yet been validated. In the present study, quantitative polymerase chain reaction was performed to quantify the relative expression of miR-142-3p in 53 paired RCC and adjacent normal tissues. Furthermore, wound healing, 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide and flow cytometry assays were performed to analyze the impacts of miR-142-3p on cellular migration, proliferation and apoptosis. The results demonstrated that miR-142-3p was significantly upregulated in RCC tissues compared with adjacent normal tissues. Downregulation of miR-142-3p, induced by chemically synthesized miR-142-3p inhibitor, significantly suppressed cell migration and proliferation, and promoted cell apoptosis in 786-O and ACHN cells, supporting the theory that miR-142-3p may function as an oncogene in RCC. The potential clinical significance of miR-142-3p, as a biomarker and therapeutic target, provides rationale for further investigation into the miR-142-3p-mediated molecular pathway and how it is associated with RCC development.