cDNA cloning and expression pattern of homolog of alpha subunit of platelet-activating factor acetylhydrolase Ib from the Chinese oak silkworm, Antheraea pernyi.
cDNA cloning and expression pattern of homolog of alpha subunit of platelet-activating factor acetylhydrolase Ib from the Chinese oak silkworm, Antheraea pernyi.
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DOI：
10.1673/031.011.14801
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Qin L
中科院分区：
文献类型：
--
作者：
Li YP;Liu YQ;Wang H;Xia RX;Shi SL;Liu X;Wang SF;Qin L
Platelet-activating factor acetylhydrolase (PAF-AH) is an enzyme that catalyzes the hydrolysis of platelet-activating factor (PAF). A homolog of alpha subunit of PAF-AH(Ib) from Antheraea pernyi (Guérin-Méneville) (Lepidoptera: Saturniidae) (ApPAFAHIbα) was isolated and characterized. The obtained cDNA sequence was 1843 base pairs (bp) long with an open reading frame (ORE) of 678 bp encoding 225 amino acids. The predicted amino acid sequence shared several conserved features of PAF-AHs of other organisms, and revealed 88, 60, and 46% identity with the homologues of Bombyx mori, Drosophila melanogaster, and Homo sapiens, respectively. Phylogenetic analysis indicated that lepidopteran PAFAHIbαs including ApPAFAHIbα might be a new member of the PAF-AHs family of insects. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis showed that the ApPAFAHIbα gene was transcribed at four developmental stages and expressed in all tissues tested.
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DOI：
10.1673/031.010.14140
发表时间：
2010
期刊：
Journal of insect science (Online)
影响因子：
--
作者：
Liu Y;Li Y;Li X;Qin L
通讯作者：
Qin L
影响因子：
2
作者：
Lee, E;Lee, SJ;Chang, HW
通讯作者：
Chang, HW
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M
影响因子：
4.8
作者：
TJOELKER, LW;EBERHARDT, C;GRAY, PW
通讯作者：
GRAY, PW
柞蚕两个烯醇化酶基因的cDNA克隆及表达模式
影响因子：
3.7
作者：
Liu, Yanqun;Li, Yuping;Xiang, Zhonghuai
通讯作者：
Xiang, Zhonghuai