Comparative whole-genome analyses reveal over 100 putative phase-variable genes in the pathogenic Neisseria spp.
Comparative whole-genome analyses reveal over 100 putative phase-variable genes in the pathogenic Neisseria spp.
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DOI：
10.1099/00221287-147-8-2321
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发表时间：
2001-08-01
期刊：
影响因子：
2.8
通讯作者：
Saunders, NJ
中科院分区：
文献类型：
--
作者：
Snyder, LAS;Butcher, SA;Saunders, NJ
此前，脑膜炎奈瑟氏菌菌株MC 58的完整基因组分析揭示了迄今为止在任何物种中描述的最大的推定相位可变基因库。与两种不完全奈瑟氏菌属的初步比较当时可获得的基因组序列揭示了与这些基因相关的重复序列的差异，这些差异表现为多态性、某些等位基因中潜在的不稳定元件的缺失以及存在的基因库。对N.脑膜炎球菌菌株Z2491和淋病奈瑟菌菌株FA 1090的基因组序列的比较分析，并结合三种可用的全基因组序列之间的综合比较分析。这增加了这些搜索的敏感性，并提供了额外的上下文信息，有利于解释重复不稳定的功能后果。结果表明：（1）在N.脑膜炎球菌Z2491株，而不是以前报道的27株; 82株淋病奈瑟氏球菌（N. gonorrhoeae）FA 1090;脑膜炎菌株MC 58，包括通过与其他两种菌株交叉比较鉴定的另外19种。除了opa基因家族的IS成员之外，还描述了119个推定的相变基因，这表明这些物种中这种基因转换机制介导的多样化具有巨大潜力，这对于它们与宿主的相互作用和环境转变至关重要。其中82个是已知的（14个）或强的（68个）相位变异的候选者，它们与opa基因一起构成总共100个鉴定的基因。在这项分析中确定的基因库偏离不同的物种分组，表明水平交换，显着影响物种和菌株互补这些基因。
Previously, a complete genome analysis of Neisseria meningitidis strain MC58 revealed the largest repertoire of putative phase-variable genes described in any species to date. initial comparisons with two incomplete Neisseria spp. genome sequences available at that time revealed differences in the repeats associated with these genes in the form of polymorphisms, the absence of the potentially unstable elements in some alleles, and in the repertoire of the genes that were present. Analyses of the complete genomes of N. meningitidis strain Z2491 and Neisseria gonorrhoeae strain FA1090 have been performed and are combined with a comprehensive comparative analysis between the three available complete genome sequences. This has increased the sensitivity of these searches and provided additional contextual information that facilitates the interpretation of the functional consequences of repeat instability. This analysis identified: (i) 68 phase-variable gene candidates in N. meningitidis strain Z2491, rather than the 27 previously reported; (iii) 83 candidates in N. gonorrhoeae strain FA1090; and (iiii) 82 candidates in N. meningitidis strain MC58, including an additional 19 identified through cross-comparisons with the other two strains. In addition to the IS members of the opa gene family, a repertoire of 119 putative phase-variable genes is described, indicating a huge potential for diversification mediated by this mechanism of gene switching in these species that is central to their interactions with the host and environmental transitions. Eighty-two of these are either known (14) or strong (68) candidates for phase variation, which together with the opa genes make a total of 100 identified genes. The repertoires of the genes identified in this analysis diverge from the different species groupings, indicating horizontal exchange that significantly affects the species and strain complements of these genes.