Expression of two mRNAs encoding EGF-related proteins identifies subregions of sea urchin embryonic ectoderm.
Expression of two mRNAs encoding EGF-related proteins identifies subregions of sea urchin embryonic ectoderm.
复制标题
编码 EGF 相关蛋白的两种 mRNA 的表达可识别海胆胚胎外胚层的亚区域。
DOI：
10.1016/0012-1606(91)90053-6
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发表时间：
1991
影响因子：
2.7
通讯作者：
Angerer,LM
中科院分区：
文献类型：
--
作者：
Grimwade,JE;Gagnon,ML;Yang,Q;Angerer,RC;Angerer,LM
许多含有表皮生长因子 (EGF) 相关结构域的蛋白质在介导细胞命运规范的细胞间相互作用中发挥作用。我们使用原位杂交表明，两个 EGF 相关基因（SpEGF I 和 SpEGF II）的表达仅限于海胆幼虫的同一外胚层细胞亚群。然而，外胚层和口后面部上皮的不同上皮细胞中EGF I mRNA的浓度是恒定的，而EGF II mRNA的浓度是高度调节的。 RNase 保护测定表明，这两个基因在外胚层创始细胞建立期间（即 EGF I 和 EGF II 分别在第四次和第五次分裂之间以及第五次和第六次分裂之间）被激活。由于高转录率，到间充质囊胚阶段，EGF I mRNA 达到最大丰度（800-1000 拷贝/表达细胞），而到原肠胚阶段，EGF II mRNA 浓度达到峰值，约为该浓度的一半。 EGF I 表达开始于卵子发生的早期阶段，而 EGF II 表达似乎仅限于胚胎发生。
Many proteins containing domains related to epidermal growth factor (EGF) function in intercellular interactions that mediate specification of cell fate. We have usedin situhybridization to show that the expression of two EGF-related genes (SpEGF I and SpEGF II) is restricted to the same subset of ectodermal cells in sea urchin pluteus larvae. However, the concentration of EGF I mRNA in different epithelial cells of aboral ectoderm and postoral facial epithelium is constant while that of EGF II mRNA is highly modulated. RNase protection assays show that both genes are activated during the period when ectoderm founder cells are established, i.e., between fourth and fifth and between fifth and sixth cleavages for EGF I and EGF II, respectively. By mesenchyme blastula stage EGF I mRNA reaches maximum abundance (800–1000 copies/expressing cell) as a result of a high transcription rate, while EGF II mRNA peaks at about half that concentration by gastrula stage. EGF I expression begins at early stages of oogenesis while EGF II expression appears to be confined to embryogenesis.