Epidermal label-retaining cells: Background and recent applications
Epidermal label-retaining cells: Background and recent applications
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DOI：
10.1111/j.1087-0024.2004.09313.x
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发表时间：
2004-09-01
影响因子：
--
通讯作者：
Watt, FM
中科院分区：
文献类型：
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作者：
Braun, KM;Watt, FM
给新生小鼠反复注射~H-胸腺嘧啶核苷或5-溴-2‘-脱氧尿嘧啶核苷，然后找到成年后仍有标记的细胞，即可鉴定出表皮标记细胞(LRC)。尽管标记保留仅仅是细胞增殖史的一个标志，但有证据表明它是表皮干细胞的特征。在这里，我们回顾了早期关于LRC的文献，然后重点介绍了最近的两个应用。我们描述了如何通过整装的表皮标记来可视化LRC，以及如何使用纯化的LRC来筛选表皮干细胞隔室的标记。
Epidermal label-retaining cells (LRC) can be identified by giving neonatal mice repeated injections of 3 H-thymidine or 5-bromo-2'-deoxyuridine and then finding the cells that are still labelled in adulthood. Although label retention is simply a marker of the proliferative history of a cell, there is, nevertheless, evidence that it is a characteristic of epidermal stem cells. Here we review the early literature on LRC and then highlight two recent applications. We describe how LRC can be visualized by whole-mount labelling of the epidermis and how purified LRC can be used to screen for markers of the epidermal stem cell compartment.