Temporal Sensing Platform Based on Bipolar Electrode for the Ultrasensitive Detection of Cancer Cells
Temporal Sensing Platform Based on Bipolar Electrode for the Ultrasensitive Detection of Cancer Cells
复制标题
基于双极电极的时间传感平台用于超灵敏检测癌细胞
DOI：
10.1021/acs.analchem.6b02204
复制
发表时间：
2016
影响因子：
7.4
通讯作者：
Chen Hong-Yuan
中科院分区：
文献类型：
--
作者：
Shi Hai-Wei;Zhao Wei;Liu Zhen;Liu Xi-Cheng;Xu Jing-Juan;Chen Hong-Yuan
我们报告了一个双极电极（BPE）传感平台的时间检测癌细胞。结合阳极溶解和电化学发光（ECL）的优点，这种策略显示出超低的检测限低至5个细胞/cm 2。以阳极为报告极，通过DNA双链组装Au纳米粒子，作为鲁米诺/H2 O2电化学发光反应的催化剂和阳极溶解探针Ag化学还原的种子。银层溶解的持续时间与银的量呈正相关，但与控制电位和电路的电导率呈负相关。因此，可以通过调整其他两个因素来放大轻微的电导率变化。由于Ag@Au的形成完全淬灭了鲁米诺的ECL发射，因此ECL发射恢复反映了阳极溶解的程度。通过监测阴极上细胞孵育前后的ECL恢复时间，由于电导率的微小差异，可以定量少数细胞。这种方法有几个优点。首先，阳极溶解和ECL的组合显著提高了BPE装置的检测灵敏度。此外，该策略拓宽了BPE在癌细胞超灵敏监测中的应用，其被应用于研究抗体和适体对MCF-7和A549的捕获效率。
We report a bipolar electrode (BPE) sensing platform for the temporal detection of cancer cells. Combining the advantages of anodic dissolution and electrochemiluminescence (ECL), this strategy shows an ultralow detection limit down to 5 cells/cm2. At the anode working as the reporting pole, Au NPs were assembled through DNA double strand, which served as both catalyzer for the ECL reaction of luminol/H2O2and seeds for the chemical reduction of Ag, the anodic dissolution probe. The duration of Ag layer dissolution was positively correlated with the amount of Ag but negatively related to the controlled potential and the conductivity of the circuit. Therefore, it was possible to amplify a slight conductivity change through tuning the other two factors. As the formation of Ag@Au completely quenched the ECL emission of luminol, the ECL emission recovery reflected the extent of anodic dissolution. Through monitoring the ECL recovery time before and after the incubation of cells on the cathode, a few number of cells could be quantified due to slight difference of the conductivity. This method shows several merits. First, the combination of anodic dissolution and ECL significantly increases the detection sensitivity of BPE device. In addition, this strategy broadens the application of BPE for the ultrasensitive monitoring of cancer cells, which was applied to investigate the capture efficiencies of antibodies and aptamers toward MCF-7 and A549.