Metabonomic Analysis of the Therapeutic Effects of Chinese Medicine Sanqi Oral Solution on Rats With Exhaustive Exercise
Metabonomic Analysis of the Therapeutic Effects of Chinese Medicine Sanqi Oral Solution on Rats With Exhaustive Exercise
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DOI：
10.3389/fphar.2019.00704
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发表时间：
2019-07
影响因子：
5.6
通讯作者：
Peng Xu;Shasha Li;R. Tian;Ling Han;W. Mao;Li Li-Li;Chuang Li;Yi-ming Wang;G. Luo;N. Yang
中科院分区：
文献类型：
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作者：
Peng Xu;Shasha Li;R. Tian;Ling Han;W. Mao;Li Li-Li;Chuang Li;Yi-ming Wang;G. Luo;N. Yang
力竭运动已成为当今一个重要的健康问题。本研究旨在探讨大鼠力竭运动后代谢物的变化及中药三七口服液的整体疗效。通过力竭性游泳建立大鼠力竭运动模型。将30只雄性SD大鼠随机分为对照组、模型组和治疗组。SQ（12 mL·kg-1·d-1）或0.9%生理盐水灌胃给药。4周后，收集血清样品用于生化测量和基于超高效液相色谱（UPLC）/四极杆飞行时间质谱（Q-TOF-MS）的代谢组学研究。结果表明，参芪扶正液干预组大鼠力竭游泳时间较模型组延长（P < 0.05），参芪扶正液组平均为1160.36 ± 123.89s，模型组为906.57 ± 172.11s。在生化指标中，力竭运动大鼠肌酸激酶同工酶、乳酸脱氢酶和葡萄糖水平升高，而肌酸激酶、尿素、甘油三酯和总胆固醇水平降低。SQ给药后，除甘油三酯外，上述生化指标均正常。在血清中鉴定了属于鞘脂、磷脂、脂肪酸、氨基酸和其他类别的27种潜在生物标志物。结果表明，SQ对力竭运动具有保护作用，其作用机制可能与延长游泳耐力时间有关。基于代谢组学的代谢状态结果和血清中潜在生物标志物的分析与生化评估良好相关，证实SQ具有明确的疗效。此外，SQ对脂质代谢和糖酵解途径的影响表明，SQ具有调节能量代谢、改善信号传导和改善肌肉细胞生理功能的药理作用，可作为运动营养补充剂。
Exhaustive exercise has emerged as an important health issue nowadays. This study was designed to assess the metabolite abnormalities of rats after exhaustive exercise and the holistic efficacy of Chinese medicine Sanqi oral solution (SQ). Through exhaustive swimming, the exhaustive exercise model in rats was established. Thirty male Sprague–Dawley rats were randomly divided into control, model, and treatment groups. SQ (12 mL·kg−1·d−1) or 0.9% saline solution was administrated orally by gastric gavage. After 4 weeks, serum samples were collected for biochemical measurements and ultra performance liquid chromatography (UPLC)/quadrupole time-of-flight mass spectrometry (Q-TOF-MS)-based metabonomic study. It was found that rats with SQ intervention showed longer exhaustive swimming time (P < 0.05) than model rats, with an average of 1,160.36 ± 123.89 s in SQ group and 906.57 ± 172.11 s in model group. Among the biochemical indices, the levels of creatine kinase isoenzyme, lactate dehydrogenase, and glucose of exhaustive exercise rats increased, whereas levels of creatine kinase, urea, triglyceride, and total cholesterol decreased. These biochemical indices came normal after SQ administration, except for triglyceride. Twenty-seven potential biomarkers belonging to sphingolipids, phospholipids, fatty acids, amino acid, and other classes were identified in serum. This study indicated that SQ exerted protective effects on exhaustive exercise by significantly prolonging the swimming endurance time. The metabonomic-based findings of the metabolic state and analysis of potential biomarkers in serum well correlated with biochemical assessment, confirming that SQ had a definite efficacy. Moreover, the shifts in lipid-related metabolites and glycolytic pathway suggested that SQ may serve as a potential supplementation in sports nutrition for its pharmacological effect of regulating energy metabolism as well as improving signal transduction and muscle-cell physiological functions.