Genetic diversity of enterovirus G detected in faecal samples of wild boars in Japan: identification of novel genotypes carrying a papain-like cysteine protease sequence
Genetic diversity of enterovirus G detected in faecal samples of wild boars in Japan: identification of novel genotypes carrying a papain-like cysteine protease sequence
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DOI：
10.1099/jgv.0.001446
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发表时间：
2020-01-01
影响因子：
3.8
通讯作者：
Nagai, Makoto
中科院分区：
文献类型：
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作者：
Nagata, Ayaka;Sekiguchi, Yuya;Nagai, Makoto
对日本野猪肠道病毒G （EV-G）的遗传多样性进行了研究。80份粪便样本中有30份（37.5%）呈ev - g特异性逆转录PCR阳性。其中，20份样本的病毒蛋白1 （VP1）片段分为G1（3份）、G4（1份）。G6（2个样本），G8（4个样本），G11（1个样本）。G12（7个样本）。G14（1个样本）和G17（1个样本）中有11个样本含有木瓜蛋白酶样半胱氨酸蛋白酶（PL-CP）序列，认为这是在61个（2个样本）或G17个（1个样本）野猪EV-Gs中首次发现，在G8（2个样本）或G12个（6个样本）的EV-Gs中首次发现。具有PL-CP序列的EV-Gs （PL-CP EV-Gs）不论基因型或来源，其非结构蛋白区域序列相似，表明这些菌株存在共同的祖先。有趣的是，对于两个G8和两个G12样本，基因组序列包含两个版本，有或没有PL-CP序列。与同源的2C/PL-CP和PL-CP/3A连接序列一起，这可能解释了PL-CP EV-G基因组中PL-CP序列的重组和缺失。这些发现揭示了EV-G的遗传可塑性和进化。
The genetic diversity of enterovirus G (EV-G) was investigated in the wild-boar population in Japan. EV-G-specific reverse transcription PCR demonstrated 30 (37.5%) positives out of 80 faecal samples. Of these, viral protein 1 (VP1) fragments of 20 samples were classified into G1 (3 samples), G4 (1 sample). G6 (2 samples), G8 (4 samples), G11 (1 sample). G12 (7 samples). G14 (1 sample) and G17 (1 sample), among which 11 samples had a papain-like cysteine protease (PL-CP) sequence, believed to be the first discoveries in 61 (2 samples) or G17 (1 sample) wild-boar EV-Gs, and in G8 (2 samples) or G12 (6 samples) EV-Gs from any animals. Sequences of the non-structural protein regions were similar among EV-Gs possessing the PL-CP sequence (PL-CP EV-Gs) regardless of genotype or origin, suggesting the existence of a common ancestor for these strains. Interestingly, for the two G8 and two G12 samples, the genome sequences contained two versions, with or without the PL-CP sequence. together with the homologous 2C/PL-CP and PL-CP/3A junction sequences, which may explain how the recombination and deletion of the PL-CP sequences occured in the PL-CP EV-G genomes. These findings shed light on the genetic plasticity and evolution of EV-G.