Caenorhabditis elegans Chk2-like gene is essential for meiosis but dispensable for DNA repair
Caenorhabditis elegans Chk2-like gene is essential for meiosis but dispensable for DNA repair
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DOI：
10.1016/s0014-5793(00)02178-5
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发表时间：
2000-11-17
期刊：
影响因子：
3.5
通讯作者：
Takahashi, H
中科院分区：
文献类型：
--
作者：
Higashitani, A;Aoki, H;Takahashi, H
在秀丽隐杆线虫基因组中发现了一个chk2样基因。推测的基因产物被称为Ce-chk-2，由450个氨基酸残基组成，与Chk2/Cds1基因家族具有良好的同源性。rna介导干扰（RNAi）的结果表明，注射dsRNA的动物F1代生长到成年，但大约95%的卵（F2）在早期胚胎发生时死亡，在少数存活的后代中，雄性（XO动物）出现的频率异常高（30%）。此外，在F1的完全成熟卵母细胞中观察到12个单价基因，而在温和型卵母细胞中正常观察到6个二价基因，Ce-chk-2基因在成年期表达增加，在glp-4突变体中表达水平下降，在种系增殖缺陷中。携带Ce-chk-2 RNAi的F1胚胎的辐射敏感性未受显著影响。(C) 2000年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
A Chk2-like gene was identified in the genome of Caenorhabditis elegans. The putative gene product, termed Ce-chk-2 consists of 450 amino acid residues, and shows good homology with the Chk2/Cds1 gene family. The results of RNA-mediated interference (RNAi) indicated that the F1 generation from dsRNA injected animals grew to adulthood, but approximately 95% of their eggs (F2) died during early embryogenesis, Among the few surviving progeny, males (XO animals) arose at an abnormally high frequency (30%). In addition, 12 univalents were observed in full grown oocytes of the F1, while six bivalents were normally observed in mild-type oocytes, Ce-chk-2 gene expression increased in the adult stage, and their expression level decreased in the glp-4 mutant, which is defective in germ line proliferation. The radiation sensitivity of F1 embryos carrying Ce-chk-2 RNAi was not significantly affected. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.