Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, Haliotis discus hannai
Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, Haliotis discus hannai
复制标题
DOI：
10.1111/j.1755-0998.2008.02118.x
复制
发表时间：
2008-09
影响因子：
7.7
通讯作者：
Haigang Qi;Xiao Liu;Guo-fan Zhang
中科院分区：
文献类型：
--
作者：
Haigang Qi;Xiao Liu;Guo-fan Zhang
我们在此首次报道了商业上重要的腹足动物太平洋鲍鱼（Haliotis Discus hannai）中的 12 个多态性单核苷酸多态性 (SNP)，这些多态性是通过搜索表达序列标签数据库来鉴定的。根据四引物等位基因特异性聚合酶链反应分析，这些 SNP 位点（7 个核 SNP 和 5 个线粒体 SNP）在 37 个野生鲍鱼个体中存在多态性。所有位点都有两个等位基因，次要等位基因频率范围为 0.027 至 0.473。对于七个核 SNP，预期和观察到的杂合度范围分别为 0.053 至 0.499 和 0.054 至 0.811。
We report here for the first time 12 polymorphic single nucleotide polymorphisms (SNPs) in a commercially important gastropod, Pacific abalone (Haliotis discus hannai) that were identified by searching expressed sequence tag database. These SNP loci (seven nuclear and five mitochondrial SNPs) were polymorphic among 37 wild abalone individuals, based on a four‐primer allele‐specific polymerase chain reaction analysis. All loci had two alleles and the minor allele frequency ranged from 0.027 to 0.473. For the seven nuclear SNPs, the expected and observed heterozygosities ranged from 0.053 to 0.499 and from 0.054 to 0.811, respectively.