Continuous cell washing and mixing driven by an ultrasound standing wave within a microfluidic channel
Continuous cell washing and mixing driven by an ultrasound standing wave within a microfluidic channel
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DOI：
10.1039/b408045a
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发表时间：
2004-01-01
期刊：
影响因子：
6.1
通讯作者：
Coakley, WT
中科院分区：
文献类型：
--
作者：
Hawkes, JJ;Barber, RW;Coakley, WT
超声驻波辐射力和层流已被用来转移酵母细胞从一个液体介质到另一个（洗涤）通过连续场流分级（FFF）的方法。平行于3 MHz驻波谐振器的节平面引入两个共流流，无细胞悬浮相（流速>总流通体积的50%）和酵母悬浮液。谐振器被制造成在腔室的中心线处具有单个压力节面。层流确保了当两个悬浮相流过声场时保持稳定的界面。超声的启动在0.5s内将细胞转移到无细胞相。该颗粒转移程序避免了基于离心的洗涤程序的沉淀形成和再悬浮步骤。此外，流体混合证明在同一个腔室在较高的声压。该通道在可忽略的背压下运行（横截面，0.25 x 10 mm），并且仅具有一个流动会聚和一个流动分离步骤，该通道不易堵塞。作用在细胞上的力很小;小于在产生100 g的离心机中经历的力。所描述的声学驱动的细胞转移和混合程序可能特别适合于分子生物学和微生物学中所需的日益复杂的操作，特别是适合于它们向连续流动过程的转化。
Ultrasound standing wave radiation force and laminar flow have been used to transfer yeast cells from one liquid medium to another (washing) by a continuous field-flow fractionation (FFF) approach. Two co-flowing streams, a cell-free suspending phase (flow rate > 50% of the total flow-through volume) and a yeast suspension, were introduced parallel to the nodal plane of a 3 MHz standing wave resonator. The resonator was fabricated to have a single pressure nodal plane at the centre line of the chamber. Laminar flow ensured a stable interface was maintained as the two suspending phases flowed through the sound field. Initiation of the ultrasound transferred cells to the cell-free phase within 0.5 s. This particle transfer procedure circumvents the pellet formation and re-suspension steps of centrifuge based washing procedures. In addition, fluid mixing was demonstrated in the same chamber at higher sound pressures. The channel operates under negligible backpressure (cross-section, 0.25 x 10 mm) and with only one flow convergence and one flow division step, the channel cannot be easily blocked. The force acting on the cells is small; less than that experienced in a centrifuge generating 100g. The acoustically-driven cell transfer and mixing procedures described may be particularly appropriate for the increasingly complex operations required in molecular biology and microbiology and especially for their conversion to continuous flow processes.