Different Serum Free Fatty Acid Profiles in NAFLD Subjects and Healthy Controls after Oral Fat Load.
Different Serum Free Fatty Acid Profiles in NAFLD Subjects and Healthy Controls after Oral Fat Load.
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DOI：
10.3390/ijms17040479
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发表时间：
2016-03-31
影响因子：
5.6
通讯作者：
Cassader M
中科院分区：
文献类型：
--
作者：
Gambino R;Bugianesi E;Rosso C;Mezzabotta L;Pinach S;Alemanno N;Saba F;Cassader M
背景：游离脂肪酸（FFA）代谢可影响代谢状况，如肥胖和非酒精性脂肪性肝病（NAFLD）。这项工作研究了NAFLD受试者中总游离脂肪酸的增加，以可能表征哪些脂肪酸显著地占了整个增加。方法：选择21例NAFLD患者。对照组由9名健康受试者组成。所有受试者都接受了口服标准脂肪负荷。甘油三酸酯;胆固醇;FFA;每2 h测定一次葡萄糖和胰岛素，同时测定游离脂肪酸的脂肪酸组成。结果：NAFLD患者血清FFA水平升高主要与不同时期油酸、棕榈酸和亚油酸水平有关。二十二碳六烯酸、亚麻酸、二十碳三烯酸和花生四烯酸的含量显著增加，尽管这只是一次。在餐后阶段，稳态模型评估HOMA指数与NAFLD患者ω3/ω6比值呈正相关。结论：NAFLD患者血清游离脂肪酸水平升高主要是由于油酸和棕榈酸这两种循环游离脂肪酸含量最多。这与亚油酸的显著增加几乎完全一致。n-3/n-6脂肪酸比例的不平衡可以调节餐后反应，对胰岛素抵抗者（如NAFLD患者）的影响更为明显。
Background: Free fatty acid (FFA) metabolism can impact on metabolic conditions, such as obesity and nonalcoholic fatty liver disease (NAFLD). This work studied the increase in total FFA shown in NAFLD subjects to possibly characterize which fatty acids significantly accounted for the whole increase. Methods: 21 patients with NAFLD were selected according to specified criteria. The control group consisted of nine healthy subjects. All subjects underwent an oral standard fat load. Triglycerides; cholesterol; FFA; glucose and insulin were measured every 2 h with the determination of fatty acid composition of FFA. Results: higher serum FFA levels in NAFLD subjects are mainly due to levels of oleic, palmitic and linoleic acids at different times. Significant increases were shown for docosahexaenoic acid, linolenic acid, eicosatrienoic acid, and arachidonic acid, although this was just on one occasion. In the postprandial phase, homeostatic model assessment HOMA index positively correlated with the ω3/ω6 ratio in NAFLD patients. Conclusions: the higher serum levels of FFA in NAFLD subjects are mainly due to levels of oleic and palmitic acids which are the most abundant circulating free fatty acids. This is almost exactly corresponded with significant increases in linoleic acid. An imbalance in the n-3/n-6 fatty acids ratio could modulate postprandial responses with more pronounced effects in insulin-resistant subjects, such as NAFLD patients.