Xenografted Human Amniotic Membrane-Derived Mesenchymal Stem Cells Are Immunologically Tolerated and Transdifferentiated Into Cardiomyocytes
Xenografted Human Amniotic Membrane-Derived Mesenchymal Stem Cells Are Immunologically Tolerated and Transdifferentiated Into Cardiomyocytes
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DOI：
10.1161/circresaha.109.205260
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发表时间：
2010-05-28
影响因子：
20.1
通讯作者：
Umezawa, Akihiro
中科院分区：
文献类型：
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作者：
Tsuji, Hiroko;Miyoshi, Shunichiro;Umezawa, Akihiro
基本原理：羊膜被认为具有转分化成多个器官的能力，并有望刺激减少免疫reaction.Objective：确定人羊膜间充质细胞（hAMCs）是否可以成为一个理想的同种异体移植的干细胞来源心脏再生medicine.Methods和结果：我们建立了hAMCs。体外诱导后，hAMCs自发搏动，计算的心肌转分化效率为33%。心肌梗死后2周移植hAMCs可改善超声心动图测量的受损左心室短轴缩短率（34 +/- 2% [n=8]至39 +/- 2%[n=11]; P
Rationale: Amniotic membrane is known to have the ability to transdifferentiate into multiple organs and is expected to stimulate a reduced immunologic reaction.Objective: Determine whether human amniotic membrane-derived mesenchymal cells (hAMCs) can be an ideal allograftable stem cell source for cardiac regenerative medicine.Methods and Results: We established hAMCs. After cardiomyogenic induction in vitro, hAMCs beat spontaneously, and the calculated cardiomyogenic transdifferentiation efficiency was 33%. Transplantation of hAMCs 2 weeks after myocardial infarction improved impaired left ventricular fractional shortening measured by echocardiogram (34 +/- 2% [n=8] to 39 +/- 2%[n=11]; P