Sequential Regulation of Maternal mRNAs through a Conserved cis-Acting Element in Their 3′ UTRs
Sequential Regulation of Maternal mRNAs through a Conserved cis-Acting Element in Their 3′ UTRs
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DOI：
10.1016/j.celrep.2018.12.007
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发表时间：
2018-12-26
期刊：
影响因子：
8.8
通讯作者：
Rangan, Prashanth
中科院分区：
文献类型：
--
作者：
Flora, Pooja;Wong-Deyrup, Siu Wah;Rangan, Prashanth
卵子发生过程中合成的母体 mRNA 启动后代的发育。一些母体 mRNA 要么是体细胞决定簇，要么是种系决定簇，必须在翻译上受到抑制，直到胚胎发生。然而，翻译抑制因子本身受到时间调节。我们使用果蝇母体 mRNA 极性颗粒成分 (pgc) 来探究在监管环境发生变化时母体转录本是如何受到抑制的。 pgc 是种系决定簇，在整个卵子发生过程中受到翻译调控。我们发现不同的保守RNA结合蛋白结合pgc mRNA 3' UTR中的10-nt序列，以在卵子发生的不同阶段持续抑制翻译。 Pumilio 与未分化和早期分化卵母细胞中的该序列结合，以阻断 Pgc 翻译。分化后，Bruno 水平增加，使 Bruno 能够结合相同的序列并接管 pgc mRNA 的翻译抑制。我们已经鉴定出一类受类似调控的母体 mRNA，包括合子基因组激活剂 zelda。
Maternal mRNAs synthesized during oogenesis initiate the development of future generations. Some maternal mRNAs are either somatic or germline determinants and must be translationally repressed until embryogenesis. However, the translational repressors themselves are temporally regulated. We used polar granule component (pgc), a Drosophila maternal mRNA, to ask how maternal transcripts are repressed while the regulatory landscape is shifting. pgc, a germline determinant, is translationally regulated throughout oogenesis. We find that different conserved RNA-binding proteins bind a 10-nt sequence in the 3' UTR of pgc mRNA to continuously repress translation at different stages of oogenesis. Pumilio binds to this sequence in undifferentiated and early-differentiating oocytes to block Pgc translation. After differentiation, Bruno levels increase, allowing Bruno to bind the same sequence and take over translational repression of pgc mRNA. We have identified a class of maternal mRNAs that are regulated similarly, including zelda, the activator of the zygotic genome.