Remodelling of membrane phospholipids during transition to diapause and cold-acclimation in the larvae of Chymomyza costata (Drosophilidae)
Remodelling of membrane phospholipids during transition to diapause and cold-acclimation in the larvae of Chymomyza costata (Drosophilidae)
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DOI：
10.1016/s1096-4959(03)00117-9
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发表时间：
2003-07-01
影响因子：
2.2
通讯作者：
Simek, P
中科院分区：
文献类型：
--
作者：
Kostál, V;Berková, P;Simek, P
对不同生理状态的中肋食糜蝇幼虫的脂肪体和肌肉组织中磷脂酰乙醇胺（PEs）和磷脂酰胆碱（PCs）的分子种类组成进行了分析，这些幼虫的耐冻性水平存在显着差异。积极移动和摄食的三龄幼虫具有低（零）的耐冻能力和相似的磷脂（PL）成分，无论其发育命运如何（非滞育与滞育）。在这些幼虫中发现了 PL 成分的广泛重塑，以响应：(a) 将非滞育幼虫在 5°C 冷藏 1 个月； (b) 发育过渡到滞育； (c) 滞育幼虫的冷冻。向滞育和寒冷的过渡导致耐冻性的增加。含有棕榈酸/亚油酸 (16:0/18:2) 脂肪酰基 (Eks) 酯化到甘油的 sn-1/sn-2 位点的分子种类的摩尔比例的增加是最显着的变化，这与冷冻耐受性的增加在统计上紧密相关。在脂肪体和肌肉组织的 PE 和 PC 中，16:0/18:2 FA 组合的 PL 一致增加，以响应寒冷和滞育开始。这种增加被各种分子种类的减少所抵消，具体取决于组织和脂质类别。大多数减少的物种都有一个共同的主题：它们具有在甘油的 sn-1 位上酯化的饱和 FA（棕榈基、长效甘油基、硬脂基）和在甘油的 sn-2 位上酯化的单不饱和脂肪酸（棕榈油基、油基）。讨论了 PL 分子物种重塑的可能适应性意义。 (C) 2003 Elsevier Science Inc. 保留所有权利。
The composition of molecular species of phosphatidylethanolamines (PEs) and phosphatidylcholines (PCs) was analysed in fat body and muscle tissues of Chymomyza costata larvae of different physiological states that markedly differed in their level of freeze-tolerance. Actively moving and feeding 3rd instar larvae had low (zero) capacity of freeze-tolerance and similar phospholipid (PL) compositions irrespective of their developmental destiny (non-diapause vs. diapause). Extensive remodelling of PL composition was found in these larvae in response to: (a) chilling of non-diapause larvae at 5 degreesC for 1 month; (b) developmental transition to diapause; and (c) chilling of diapause larvae. Transition to diapause and chilling led to an increase in freeze-tolerance. The increase in molar proportion of molecular species containing palmitic/linoleic (16:0/18:2) fatty acyls (Eks) esterified to sn-1/sn-2 positions of glycerol was the most prominent change, which was tightly statistically correlated with increasing freeze-tolerance. The increase of PLs with combination of 16:0/18:2 FAs was registered consistently in PEs and PCs in fat body and muscle tissues in response to chilling and to diapause onset. This increase was countered by a decreases of various molecular species, depending on tissue and lipid class. Most decreasing species shared one common theme: they had a saturated FA (palmityl, margaryl, stearyl) esterified at sn-1 position and a monounsaturate (palmitoleyl, oleyl) esterified at sn-2 position of glycerol. Possible adaptive meaning of PL molecular species remodelling is discussed. (C) 2003 Elsevier Science Inc. All rights reserved.