Genotyping and Molecular Characterization of Hepatitis B Virus from Human Immunodeficiency Virus-Infected Individuals in Southern Africa
Genotyping and Molecular Characterization of Hepatitis B Virus from Human Immunodeficiency Virus-Infected Individuals in Southern Africa
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DOI：
10.1371/journal.pone.0046345
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发表时间：
2012-09-28
期刊：
影响因子：
3.7
通讯作者：
Kramvis, Anna
中科院分区：
文献类型：
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作者：
Makondo, Euphodia;Bell, Trevor G.;Kramvis, Anna
乙型肝炎病毒（HBV）和人类免疫缺陷病毒（HIV）在撒哈拉以南非洲是高地方性的。在感染艾滋病毒的非洲人中流行的HBV基因型尚不清楚。我们的目的是确定hiv感染参与者的HBV基因型，并确定临床显著的HBV突变。从71例HBV DNA(+ve) hiv感染者中，成功测序了49个基本核心启动子/前核（BCP/PC）和29个完整S区。对完整S区29份标本进行系统发育分析，其中28份属于A1亚基因型，1份属于D3亚基因型。在转录（1762T1764A），翻译（Kozak 1809-1812，起始1814-1816，G1896A与C1858T）或翻译后水平（G1862T）影响HBeAg表达的突变是观察到的高HBeAg阴性的原因。G1862T突变仅发生在A1亚基因型分离株中，在三分之一（7/21）的HBsAg（-ve）参与者中发现，但在18名HBsAg（+ve）参与者中没有发现
Hepatitis B virus (HBV) and human immunodeficiency virus (HIV) are hyperendemic in sub-Saharan Africa. The HBV genotypes prevailing in HIV-infected Africans are unknown. Our aim was to determine the HBV genotypes in HIV-infected participants and to identify clinically significant HBV mutations. From 71 HBV DNA(+ve) HIV-infected participants, 49 basic core promoter/precore (BCP/PC) and 29 complete S regions were successfully sequenced. Following phylogenetic analysis of 29 specimens in the complete S region, 28 belonged to subgenotype A1 and one to D3. Mutations affecting HBeAg expression at the transcriptional (1762T1764A), translational (Kozak 1809-1812, initiation 1814-1816, G1896A with C1858T), or post translational levels (G1862T), were responsible for the high HBeAg-negativity observed. The G1862T mutation occurred only in subgenotype A1 isolates, which were found in one third (7/21) of HBsAg(-ve) participants, but in none of the 18 HBsAg(+ve) participants (p