Two Intracellular Symbiotic Bacteria from the Mulberry Psyllid Anomoneura mori (Insecta, Homoptera)
Two Intracellular Symbiotic Bacteria from the Mulberry Psyllid Anomoneura mori (Insecta, Homoptera)
复制标题
DOI：
10.1128/aem.64.10.3599-3606.1998
复制
发表时间：
1998-10
影响因子：
4.4
通讯作者：
T. Fukatsu;N. Nikoh
中科院分区：
文献类型：
--
作者：
T. Fukatsu;N. Nikoh
摘要采用分子系统发育分析和原位杂交相结合的方法，对桑葚木虱Anomoneura mori胞内共生菌进行了鉴定。在木虱的腹部，有一个大的、黄色的、两裂的菌囊（或细菌囊），它由许多圆形的单核菌细胞（或细菌细胞）包围着合胞体组织。菌胞和合胞体分别具有特定的细胞内细菌，即X-共生体和Y-共生体。从A.用限制性片段长度多态性（RFLP）分析鉴定了A型和B型两个克隆。用特异性寡核苷酸探针进行的原位杂交表明，A型和B型16 SrDNA分别来自X型和Y型共生菌。16 S rDNA序列的分子系统发育分析表明，这些共生菌属于变形菌门γ亚门中的不同谱系。在数据库中没有16 S rDNA序列与X-和Y-共生体的16 S rDNA序列密切相关。然而，与它们关系比较密切的序列是其他昆虫内共生体的序列。X-和Y-共生体的16 S rDNA的核苷酸组成是高度AT偏置的，并且X-共生体的序列是迄今为止报道的最富含AT的细菌16 S rDNA序列。
ABSTRACT We characterized the intracellular symbiotic bacteria of the mulberry psyllid Anomoneura mori by performing a molecular phylogenetic analysis combined with in situ hybridization. In its abdomen, the psyllid has a large, yellow, bilobed mycetome (or bacteriome) which consists of many round uninucleated mycetocytes (or bacteriocytes) enclosing syncytial tissue. The mycetocytes and syncytium harbor specific intracellular bacteria, the X-symbionts and Y-symbionts, respectively. Almost the entire length of the bacterial 16S ribosomal DNA (rDNA) was amplified and cloned from the whole DNA ofA. mori, and two clones, the A-type and B-type clones, were identified by restriction fragment length polymorphism analysis. In situ hybridization with specific oligonucleotide probes demonstrated that the A-type and B-type 16S rDNAs were derived from the X-symbionts and Y-symbionts, respectively. Molecular phylogenetic analyses of the 16S rDNA sequences showed that these symbionts belong to distinct lineages in the γ subdivision of the Proteobacteria. No 16S rDNA sequences in the databases were closely related to the 16S rDNA sequences of the X- and Y-symbionts. However, the sequences that were relatively closely related to them were the sequences of endosymbionts of other insects. The nucleotide compositions of the 16S rDNAs of the X- and Y-symbionts were highly AT biased, and the sequence of the X-symbiont was the most AT-rich bacterial 16S rDNA sequence reported so far.