ORF3 protein of hepatitis E virus is not required for replication, virion assembly, or infection of hepatoma cells in vitro
ORF3 protein of hepatitis E virus is not required for replication, virion assembly, or infection of hepatoma cells in vitro
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DOI：
10.1128/jvi.00892-06
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发表时间：
2006-11-01
影响因子：
5.4
通讯作者：
Purcell, Robert H.
中科院分区：
文献类型：
--
作者：
Emerson, Suzanne U.;Nguyen, Hanh;Purcell, Robert H.
发现了一个能够相对有效地转染和感染戊型肝炎病毒的Huh-7细胞亚克隆。转染后，产生感染性病毒，但主要仍与细胞相关。细胞内病毒，通过裂解转染细胞回收，感染初始细胞。这种体外产生的病毒在抗原性上似乎与从临床样本中分离的病毒相同。转染了无法合成ORF3蛋白的突变病毒基因组的细胞裂解物中含有感染性病毒粒子，这些病毒粒子在数量、热稳定性和沉降特性上与转染了野生型病毒基因组的裂解物相似。因此，在体外感染Huh-7细胞或产生感染性病毒不需要ORF3蛋白，而在体内不需要ORF3蛋白。
A subclone of Huh-7 cells that could be relatively efficiently transfected and infected with hepatitis E virus was identified. Following transfection, infectious virus was produced but remained predominantly cell associated. Intracellular virus, recovered by lysis of transfected cells, infected naive cells. This in vitro-produced virus appeared to be antigenically identical to virus isolated from clinical samples. Lysates from cells transfected with mutant viral genomes unable to synthesize ORF3 protein contained infectious virions that were similar in number, thermostability, and sedimentation characteristics to those in lysates transfected with wild-type viral genomes. Therefore, in contrast to its requirement in vivo, ORF3 protein is not required for infection of Huh-7 cells or production of infectious virus in vitro.