COMPLETE SEQUENCE OF THE 43-KB HUMAN RIBOSOMAL DNA REPEAT - ANALYSIS OF THE INTERGENIC SPACER
COMPLETE SEQUENCE OF THE 43-KB HUMAN RIBOSOMAL DNA REPEAT - ANALYSIS OF THE INTERGENIC SPACER
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DOI：
10.1006/geno.1995.1049
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发表时间：
1995-05-20
期刊：
影响因子：
4.4
通讯作者：
SYLVESTER, JE
中科院分区：
文献类型：
--
作者：
GONZALEZ, IL;SYLVESTER, JE
我们已经对30 kb的人类核糖体DNA基因间间隔（IGS）剩余的12.4 kb进行了测序，这使我们能够拼凑出一个完整的IGS和一个43 kb的rDNA单元。完整的IGS序列揭示了一系列序列基序，这些基序可能与已知或预期存在于rDNA重复中的功能相关：转录调节、DNA复制的重组起始和染色体组织。最后，我们发现IGS的变异积累速率远高于转录区。这一发现使我们将序列特征与突变类型和序列背景与突变率联系起来。(C) 1995学术出版社，Inc。
We have sequenced the remaining 12.4 kb of the 30-kb human ribosomal DNA intergenic spacer (IGS), which allows us to piece together both a complete IGS and a 43-kb rDNA unit. The sequence of the complete IGS reveals a collection of sequence motifs that can be correlated with functions known or expected to reside in the rDNA repeat: modulation of transcription, recombination initiation of DNA replication, and chromosomal organization. Finally, we find that IGS accumulates variation at a much higher rate than the transcribed regions. This finding leads us to correlate sequence character with types of mutation and sequence context with rate of mutation. (C) 1995 Academic Press, Inc.