Aberrant expression of intelectin-1 in gastric cancer: its relationship with clinicopathological features and prognosis
Aberrant expression of intelectin-1 in gastric cancer: its relationship with clinicopathological features and prognosis
复制标题
intelectin-1在胃癌中的异常表达及其与临床病理特征及预后的关系
DOI：
10.1007/s00432-011-1088-8
复制
发表时间：
2012-01-01
影响因子：
3.6
通讯作者：
Tong, Qiangsong
中科院分区：
文献类型：
--
作者：
Zheng, Liduan;Weng, Mixia;Tong, Qiangsong
人白细胞凝集素-1（human intelectin-1，ITLN-1）是一种新发现的半乳糖结合凝集素，表达于结肠杯状细胞。由于胃腺癌可以通过浸润过程发生，我们推测ITLN-1可能在胃癌中异常表达。本研究旨在探讨ITLN-1在胃癌组织中的表达及其与临床预后的关系。方法采用免疫组化方法检测196例胃癌组织中ITLN-1的表达。ITLN-1的成绩单进行了测量，通过实时定量PCR.ResultsITLN-1的表达是不存在的正常胃粘膜，而肠上皮化生地区显示ITLN-1免疫反应。142例胃癌组织中ITLN-1表达阳性（72.4%）。在20例胃癌组织中ITLN-1的表达明显高于正常胃粘膜（P< 0.001）。ITLN-1在浸润型胃癌中的表达率高于弥漫型胃癌（P= 0.003）。ITLN-1在胃癌中的阳性表达与肿瘤分化程度呈正相关（P= 0.001）和CDX 2表达与浸润深度呈负相关（P< 0.001）（P= 0.007），淋巴结转移（P= 0.001），远处转移（P= 0.014），临床分期Ki-67表达（P = 0.001）、乙酰肝素酶表达（P<0.001）与年龄、性别、肿瘤部位、肿瘤大小无关。在单因素和多因素分析中，ITLN-1是胃癌患者生存期延长的独立预后因素（P= 0.001）。结论ITLN-1在胃癌中的异常表达与临床病理特征相关，可能是预测胃癌患者预后的一个有用的预后因素。
PurposesHuman intelectin-1 (ITLN-1) is a novel identified galactose-binding lectin that is expressed in the colonic goblet cells. Since gastric adenocarcinomas can arise through a process of intestinalization, we speculate that ITLN-1 may be aberrantly expressed in gastric cancer. This study was undertaken to examine the ITLN-1 expression in gastric cancer and correlate it with clinical outcomes.MethodsOne hundred and ninety-six gastric cancer patients were evaluated for the ITLN-1 expression by immunohistochemistry. The ITLN-1 transcripts were measured by real-time quantitative PCR.ResultsITLN-1 expression was absent in normal gastric mucosa, whereas areas of intestinal metaplasia revealed ITLN-1 immunoreactivity. One hundred and forty-two gastric cancer patients (72.4%) were positive for ITLN-1 expression. In a subtotal of 20 patients, ITLN-1 transcripts were significantly enhanced in gastric cancer tissues than in normal gastric mucosa (P< 0.001). The expression rate of ITLN-1 was higher in intestinal-type carcinomas than in diffuse-type carcinomas (P= 0.003). ITLN-1 positivity in gastric cancer was positively correlated with tumor differentiation (P= 0.001) and CDX2 expression (P< 0.001), and inversely correlated with depth of invasion (P= 0.007), lymph node metastasis (P= 0.001), distant metastasis (P= 0.014), clinical stage (P= 0.006), Ki-67 expression (P= 0.001), and heparanase expression (P< 0.001), without correlation with age, gender, tumor location, or tumor size. In univariate and multivariate analyses, ITLN-1 was an independent prognostic factor for longer survival of gastric cancer patients (P= 0.001).ConclusionThe aberrant ITLN-1 expression in gastric cancer is correlated with clinicopathological features and may be a useful prognostic factor for predicting the outcomes of gastric cancer patients.