Vitamin A-deficient quail embryos have half a hindbrain and other neural defects
Vitamin A-deficient quail embryos have half a hindbrain and other neural defects
复制标题
DOI：
10.1016/s0960-9822(02)00509-2
复制
发表时间：
1996-04-01
期刊：
影响因子：
9.2
通讯作者：
Zile, M
中科院分区：
文献类型：
--
作者：
Maden, M;Gale, E;Zile, M
背景资料：视黄酸（RA）是一种形态发生活性信号分子，被认为参与几种胚胎系统的发育（基于其效果时，过量应用和事实上，它可以检测到内源性胚胎），在这里，我们采用了一种新的方法和我们;维生素A缺乏（A（-））的鹌鹑胚胎，询问这些胚胎在没有RA的情况下发育时表现出哪些缺陷，特别是神经系统。结果：我们研究了这些A（-）胚胎的解剖结构，各种基因的表达域和几种抗体的免疫反应性。除了先前记录的心血管异常外，我们还发现A（-）胚胎体节较小，耳泡发育异常，体节继续向上和向下延伸到耳泡。在中枢神经系统，我们发现神经嵴细胞需要RA才能正常发育和存活，神经管不能将任何神经突延伸到外周。使用一般的后脑形态和Hoxa-2，Hoxb-1，Hoxb-4，Krox-20和FGF-3的表达模式作为标记，我们得出结论，在中脑（菱形4-8）的分割被破坏。与此相反，背腹轴的神经管使用Shh，胰岛-1和Pax-3标记是normall.Conclusions：这些结果表明，至少有三个角色RA在中枢神经系统的发展：神经嵴的生存，神经突起的生长和后脑图案。
Background: Retinoic acid (RA) is a morphogenetically active signalling molecule thought to be involved in the development of several embryonic systems (based on its effect when applied in excess and the fact that it can be detected endogenously in embryos), Here, we adopt a novel approach and us; the vitamin A-deficient (A(-)) quail embryo to ask what defects these embryos show when they develop in the absence of RA, with particular reference to the nervous system.Results: We have examined the anatomy, the expression domains of a variety of genes and the immunoreactivity to several antibodies in these A(-) embryos. In addition to the previously documented cardiovascular abnormalities, we find that the somites are smaller in A(-) embryos, otic vesicle development is abnormal and the somites continue up to and underneath the otic vesicle, In the central nervous system, we find that neural crest cells need RA for normal development and survival, and the neural tube fails to extend any neurites into the periphery. Using general hindbrain morphology and the expression patterns of Hoxa-2, Hoxb-1, Hoxb-4, Krox-20 and FGF-3 as markers, we conclude that segmentation in the myelencephalon (rhombomeres 4-8) is disrupted. In contrast, the dorsoventral axis of the neural tube using Shh, islet-1 and Pax-3 as markers is normal.Conclusions: These results demonstrate at least three roles for RA in central nervous system development: neural crest survival, neurite outgrowth and hindbrain patterning.