Expression levels of complement regulatory proteins (CD35, CD55 and CD59) on peripheral blood cells of patients with chronic kidney disease
Expression levels of complement regulatory proteins (CD35, CD55 and CD59) on peripheral blood cells of patients with chronic kidney disease
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DOI：
10.2147/ijgm.s216989
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发表时间：
2019-01-01
影响因子：
2.3
通讯作者：
Abd El Haliem, Naglaa F.
中科院分区：
文献类型：
--
作者：
Eldewi, Dalia Mahmoud;Alhabibi, Alshaymaa M.;Abd El Haliem, Naglaa F.
背景：补体系统调节的改变与多种肾脏疾病有关。CD35、CD55和CD59调节补体系统，它们表达的变化以前被认为与肾脏疾病有关。为了解慢性肾脏病(CKD)的发病机制，本研究探讨这些蛋白表达水平的变化是否与慢性肾脏病(CKD)相关。材料和方法：将60例CKD患者和60例年龄匹配的对照组分为两组：组I(儿童患者和对照组各30例)和组II(成人患者和对照组各30例)。结果：儿童和成人CKD患者外周血红细胞CD35、CD55和CD59的表达比例均显著低于正常对照组(P
Background: Altered regulation of the complement system is associated with multiple kidney diseases. CD35, CD55 and CD59 regulate the complement system, and changes in their expression have previously been linked with kidney disease. This study assessed whether changes in the expression levels of these proteins are associated specifically with chronic kidney disease (CKD) to understand its pathogenesis.Materials and methods: Sixty CKD patients and 60 age-matched controls were enrolled and divided into two groups: Group I (n=30 pediatric patients and n=30 controls) and Group II (n=30 adult patients and n=30 controls). The expression of CD35, CD55 and CD59 on peripheral blood cells was evaluated by flow cytometry as the proportion of positive cells expressing the marker and mean fluorescence intensity (MFI), also the relation of these markers to the stage of CKD was also evaluated.Results: Pediatric and adult CKD patients had significantly lower proportion of erythrocytes expressing CD35, CD55 and CD59 than healthy controls (P