Primary Interactions of Three Quaternary Ammonium Compounds with Blastospores of Candida albicans (MEN Strain)
Primary Interactions of Three Quaternary Ammonium Compounds with Blastospores of Candida albicans (MEN Strain)
复制标题
三种季铵化合物与白色念珠菌（MEN 菌株）芽孢子的初步相互作用
DOI：
10.1023/a:1016291021552
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发表时间：
1995
影响因子：
3.7
通讯作者：
M. Shepherd
中科院分区：
文献类型：
--
作者：
L. Schep;David S. Jones;M. Shepherd
在室温下，研究了三种季铵化合物(QAC)在白色念珠菌(MEN株)双孢子表面的吸附情况。西吡氯铵和西曲胺在30秒内达到平衡摄取，而苯扎氯铵只需5分钟。这三种药物的吸附均可用朗缪尔吸附来描述，因此在胚泡表面形成了浓度依赖的药物单分子膜。由此计算出氯化十六烷基吡啶、西曲胺和苯扎氯铵在单个胚泡表面的吸附分子数分别为1.33×1012、3.17×1012和2.32×1012。这些差异很可能是由于每个QAC在芽胞表面的阳离子氮原子和伴随的亲脂部分的取向不同所致。将这些观察结果与已知的十六烷基吡啶和西曲胺的抗黏附作用联系起来，可以得出结论，用QAC覆盖胚芽孢子表面的单层不能解释观察到的黏附减少。这表明，抗黏附效应是由于与芽胞表面黏附的直接相互作用或空间位阻所致。
The absorption of three quaternary ammonium compounds (QAC), cetylpyridinium chloride, cetrimide and benzalkonium chloride, onto the surface of biastospores ofCandida albicans(MEN strain) was examined at room temperature. Equilibrium uptake occurred in less than 30 seconds for cetylpyridinium chloride and cetrimide whereas 5 min contact time was required for benzalkonium chloride. The adsorption of all three agents may be mathematically described as Langmuirian and hence a concentration-dependent formation of drug-monolayer on the surface of the blastospore occurred. From this the number of molecules adsorbed onto the surface of a single blastospore was calculated to be 1.33 × 1012, 3.17 × 1012and 2.32 × 1012for cetylpyridinium chloride, cetrimide and benzalkonium chloride, respectively. These dissimilarities are most likely due to differences in the orientations of both the cationic nitrogen atom and the accompanying lipophilic portions of each QAC at the blastospore surface. Relating these observations to the known anti-adherence effects of cetylpyridinium chloride and cetrimide, it can be concluded that monolayer coverage of the blastospore surface with QAC does not account for the observed reduced adherence. This suggests that the anti-adherence effects are due to either direct interaction with, or steric blockade of, adhesions on the blastospore surface.