Biodegradation of phthalate esters by Paracoccus kondratievae BJQ0001 isolated from Jiuqu (Baijiu fermentation starter) and identification of the ester bond hydrolysis enzyme
Biodegradation of phthalate esters by Paracoccus kondratievae BJQ0001 isolated from Jiuqu (Baijiu fermentation starter) and identification of the ester bond hydrolysis enzyme
复制标题
酒曲副球菌BJQ0001对邻苯二甲酸酯的生物降解及酯键水解酶的鉴定
DOI：
10.1016/j.envpol.2020.114506
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发表时间：
2020
影响因子：
8.9
通讯作者：
Baoguo Sun
中科院分区：
文献类型：
--
作者：
Youqiang Xu;Karim A.H.M. Minhazul;Xiaocheng Wang;Xiao Liu;Xiuting Li;Qi Meng;Hehe Li;Chengnan Zhang;Xiaotao Sun;Baoguo Sun
邻苯二甲酸酯（PAE）污染是全球日益严重的问题。从白酒发酵剂中分离到一株对邻苯二甲酸酯（PAEs）具有良好降解能力的孔氏副球菌（Paracoccus kondratievae）BJQ 0001。据我们所知，这是第一个报告的P. kondratievae菌株能够降解邻苯二甲酸酯。第一个完整的Kondratievae P. kondratievae的基因组序列，没有缺口，由两个环状染色体和一个质粒组成。该菌能同时降解邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二乙酯（DEP）、邻苯二甲酸二丁酯（DBP）、邻苯二甲酸二异丁酯（DIBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP），且以DBP和DEP为优先降解底物。当初始浓度为200 mg/L时，半衰期（t1/2）仅为6.34 h。结合基因注释和代谢中间产物分析，提出了该物种的代谢途径。苯甲酸是PAE厌氧代谢的中间产物，在好氧降解过程中被发现。得到两个水解烷基酯键的关键酶，分别属于水解酶家族IV和VI。这些结果将促进孔氏毕赤酵母降解邻苯二甲酸酯的研究，并为提高食品质量控制和环境邻苯二甲酸酯处理提供有用的信息。
Phthalate ester (PAE) pollution is an increasing problem globally. Paracoccus kondratievae BJQ0001 was isolated from the fermentation starter of Baijiu and showed an efficient degradation capability toward PAEs. To our poor knowledge, this is the first report of a P. kondratievae strain capable of degrading PAEs. The first complete genome sequence of P. kondratievae was presented without gaps, and composed of two circular chromosomes and one plasmid. The species simultaneously degraded di-methyl phthalate (DMP), di-ethyl phthalate (DEP), di-butyl phthalate (DBP), di-isobutyl phthalate (DIBP) and di-(2-ethylhexyl) phthalate (DEHP), with DMP and DEP as the preferred substrates. The half-life (t1/2) of DMP was only 6.34 h with an initial concentration of 200 mg/L. Combined with gene annotation and metabolic intermediate analysis, a metabolic pathway was proposed for the species. Benzoic acid, the intermediate of anaerobic PAE metabolism, was identified in the aerobic degradation process. Two key enzymes for alkyl ester bond hydrolysis were obtained, and belonged to families IV and VI of hydrolases, respectively. These results will promote the investigation of PAE degradation by P. kondratievae, and provide useful information for improving the quality control of food and environmental PAE treatment.