Flow Cytometry: An Overview.
Flow Cytometry: An Overview.
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DOI：
10.1002/cpim.40
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发表时间：
2018-02-21
影响因子：
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通讯作者：
McKinnon KM
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文献类型：
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作者：
McKinnon KM
流式细胞术是一种提供溶液中单细胞的快速多参数分析的技术。流式细胞仪利用激光作为光源以产生散射光和荧光信号，所述散射光和荧光信号由诸如光电二极管或光电倍增管的检测器读取。这些信号被转换成电子信号，由计算机分析并写入标准化格式（.fcs）数据文件。可以基于细胞群的荧光或光散射特性来分析和/或纯化细胞群。在流式细胞术中使用多种荧光试剂。这些包括荧光缀合的抗体、DNA结合染料、活力染料、离子指示剂染料和荧光表达蛋白。流式细胞术是一种强大的工具，在免疫学、分子生物学、细菌学、病毒学、癌症生物学和传染病监测中具有应用。在过去的30年里，它取得了巨大的进步，使免疫系统和细胞生物学其他领域的研究获得了前所未有的细节。
Flow cytometry is a technology that provides rapid multi-parametric analysis of single cells in solution. Flow cytometers utilize lasers as light sources to produce both scattered and fluorescent light signals that are read by detectors such as photodiodes or photomultiplier tubes. These signals are converted into electronic signals that are analyzed by a computer and written to a standardized format (.fcs) data file. Cell populations can be analyzed and/or purified based on their fluorescent or light scattering characteristics. A variety of fluorescent reagents are utilized in flow cytometry. These include, fluorescently conjugated antibodies, DNA binding dyes, viability dyes, ion indicator dyes and fluorescent expression proteins. Flow cytometry is a powerful tool that has applications in immunology, molecular biology, bacteriology, virology, cancer biology and infectious disease monitoring. It has seen dramatic advances over the last 30 years, allowing unprecedented detail in studies of the immune system and other areas of cell biology.