IMMUNOCYTOCHEMICAL LOCALIZATION OF AROMATASE IN OVARIES OF SOME RODENTS, COW AND HUMAN
IMMUNOCYTOCHEMICAL LOCALIZATION OF AROMATASE IN OVARIES OF SOME RODENTS, COW AND HUMAN
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一些啮齿动物、牛和人类卵巢中芳香酶的免疫细胞化学定位
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
Y. Osawa
中科院分区：
文献类型：
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作者：
H. Matsuda;H. Fujita;K. Ishimura;Y. Osawa
为了明确芳香化酶在大鼠、小鼠、豚鼠、金黄地鼠、牛和人卵巢组织中的定位，用抗人胎盘芳香化酶Ⅱ细胞色素P-450抗体，用PAP法或间接荧光抗体法进行了免疫细胞化学研究。以及未成熟和成熟啮齿动物卵巢的间质腺细胞和成熟啮齿动物卵巢的黄体细胞。牛和人卵巢内膜细胞和黄体细胞也呈免疫反应性。只有在大的排卵前卵泡中，颗粒细胞才能与该酶的抗体发生反应，而其他卵泡中的颗粒细胞均呈阴性反应，这些结果可能暗示了内膜细胞和间质腺细胞在成熟和未成熟的卵巢中可能具有将雄激素芳构化为雌激素的基本能力。
In order to clarify the in vivo localization of aromatase in the ovarian tissues of rat, mouse, guinea pig, golden hamster, cow and human, immunocytochemical investigations using the anti-human placental aromatase II cytochrome P-450 antibody were carried out by the PAP or indirect fluorescent antibody method.Immunoreactive aromatase was detected on the theca interna, as well as the interstitial gland cells of both immature and mature rodent ovaries and the corpus luteum cell of mature rodent ovaries. The immunoreactivity was also demonstrated on the theca interna cell and corpus luteum cell of cow and human ovaries. Only in large preovulatory follicles of the mature rodent ovary did the granulosa cell react with the antibody of this enzyme, while granulosa cells in all the other follicles were consistently negative in this immunoreaction.These results might imply that the theca interna cell and interstitial gland cell presumably have a fundamental ability of the aromatization from androgens to estrogens in the mature and immature ovaries.
妊娠期雌激素生物合成的物种特异性。
DOI：
10.1016/0022-4731(81)90313-7
发表时间：
1981
期刊：
Journal of steroid biochemistry
影响因子：
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作者：
Osawa,Y;Higashiyama,T;Nakamura,T
通讯作者：
Nakamura,T