Mutations of human dihydrofolate reductase causing decreased inhibition by methotrexate.
Mutations of human dihydrofolate reductase causing decreased inhibition by methotrexate.
复制标题
人二氢叶酸还原酶的突变导致甲氨蝶呤的抑制作用减弱。
DOI：
10.1007/978-1-4615-2960-6_96
复制
发表时间：
1993
影响因子：
--
通讯作者：
Harris,SE
中科院分区：
文献类型：
--
作者：
Blakley,RL;Appleman,JR;Chunduru,SK;Nakano,T;Lewis,WS;Harris,SE
寡核苷酸定向诱变已被许多实验室用于获得关于各种酶的结构特征的重要性的信息。当一个氨基酸残基或一组残基从酶结构中缺失，或通过定向诱变被其他残基取代时，可以得出关于该结构部分与酶催化活性的关系的推论。这种方法已被广泛应用于研究二氢叶酸还原酶（DHFR）的Benkovic和他的合作者。
Oligonucleotide-directed mutagenesis has been used by many laboratories to obtain information regarding the significance of the structural features of various enzymes. When an amino acid residue, or group of residues, is deleted from the enzyme structure, or is replaced by other residues by directed mutagenesis, inferences can be drawn regarding the relation of that portion of the structure to the catalytic activity of the enzyme. This approach has been applied extensively to the study of dihydrofolate reductase (DHFR) by Benkovic and his collaborators.