Stimuli-Triggered Growth and Removal of a Bioreducible Nanoshell on Nanoparticles
Stimuli-Triggered Growth and Removal of a Bioreducible Nanoshell on Nanoparticles
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纳米颗粒上生物可还原纳米壳的刺激触发生长和去除
DOI：
10.1002/marc.201300885
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发表时间：
2014-03-01
影响因子：
4.6
通讯作者：
You, Ye-Zi
中科院分区：
文献类型：
--
作者：
Han, Li-Fen;Chen, Qian-Bao;You, Ye-Zi
报道了一种新的简单的刺激生长和去除纳米颗粒表面生物可还原纳米壳的方法。结果表明，pH或温度均能诱导含二硫化物的支化聚合物在纳米颗粒表面发生聚集，聚合态聚合物发生分子间二硫键交换，使聚合态聚合物发生交联，在纳米颗粒周围形成一层可生物还原的聚合物外壳。当用谷胱甘肽或L二硫苏糖醇处理这些具有聚合物外壳的纳米颗粒时，聚合物外壳可以很容易地从纳米颗粒中去除。这种方法的潜在应用是容易地从脂质体中生长和去除生物可还原的外壳，并提高具有生物可还原的聚乙二醇壳的脂质体的体内基因转染活性。
A new and easy method of stimuli-triggered growth and removal of a bioreducible nanoshell on nanoparticles is reported. The results show that pH or temperature could induce the aggregation of disulfide-contained branched polymers at the surface of nanoparticles; subsequently, the aggregated polymers could undergo intermolecular disulfide exchange to cross-link the aggregated polymers, forming a bioreducible polymer shell around nanoparticles. When these nanoparticles with a polymer shell are treated with glutathione (GSH) or d,l-dithiothreitol (DTT), the polymer shell could be easily removed from the nanoparticles. The potential application of this method is demonstrated by easily growing and removing a bioreducible shell from liposomes, and improvement of in vivo gene transfection activity of liposomes with a bioreducible PEG shell.