Faecalibaculum rodentium gen. nov., sp nov., isolated from the faeces of a laboratory mouse
Faecalibaculum rodentium gen. nov., sp nov., isolated from the faeces of a laboratory mouse
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DOI：
10.1007/s10482-015-0583-3
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发表时间：
2015-12-01
影响因子：
2.6
通讯作者：
Kim, Byoung-Chan
中科院分区：
文献类型：
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作者：
Chang, Dong-Ho;Rhee, Moon-Soo;Kim, Byoung-Chan
从小鼠粪便中分离出一种新型严格厌氧菌株 ALO17(T)，并发现其产生乳酸作为主要代谢终产物。观察到分离物为革兰氏染色阳性、不活动、不形成孢子的小棒、氧化酶和过氧化氢酶阴性，并且在DSM 104琼脂平板上形成奶油色菌落。生长的 NaCl 范围确定为 0-2% (w/v)。发现该分离物在 37°C、0.5%（w/v）氯化钠和 pH 7 的条件下生长最佳。发现细胞壁水解产物含有核糖作为主要糖。基因组DNA G+C含量测定为52.3mol%。 16S rRNA 基因序列的系统发育分析显示，Holdemanella biformis DSM 3989(T)、Faecalicoccus pleomorphus ATCC 29734(T)、Faecalitalea cylindroides ATCC 27803(T) 和 Allobaculum stercoricanis DSM 13633(T) 与该分离株密切相关 (87.4，序列相似性分别为 87.3%、86.9% 和 86.9%）。分离物的主要细胞脂肪酸 (> 10%) 被鉴定为 C-18:1 cis 9 FAME (36.9%)、C-16:0 FAME (33.7%) 和 C-18:0 FAME (13.2%)。与测试的参考菌株相比，仅在菌株 ALO17(T) 中检测到 C-20:0 FAME (4.0%)，而不存在 C-16:0 DMA。该分离株的底物氧化谱也与参考菌株不同，其对 d-蜜二糖和水苏糖呈阳性，但对 N-乙酰基-d-半乳糖胺和 3-甲基-d-葡萄糖呈阴性。根据本研究的多相分类学证据，该分离株被推断属于丹毒科中的一个新属。我们建议将其命名为 Faecalibaculum rodentium gen。十一月，SP。十一月适应菌株 ALO17(T) (=KCTC 15484(T) = JCM 30274(T)) 作为模式菌株。
A novel strictly anaerobic strain, ALO17(T), was isolated from mouse faeces and found to produce lactic acid as a major metabolic end product. The isolate was observed to be Gram-stain positive, non-motile, non-spore forming small rods, oxidase and catalase negative, and to form cream-coloured colonies on DSM 104 agar plates. The NaCl range for growth was determined to be 0-2 % (w/v). The isolate was found to grow optimally at 37 A degrees C, with 0.5 % (w/v) NaCl and at pH 7. The cell wall hydrolysates were found to contain ribose as a major sugar. The genomic DNA G+C content was determined to be 52.3 mol%. A phylogenetic analysis of the 16S rRNA gene sequence revealed that Holdemanella biformis DSM 3989(T), Faecalicoccus pleomorphus ATCC 29734(T), Faecalitalea cylindroides ATCC 27803(T), and Allobaculum stercoricanis DSM 13633(T) are closely related to the isolate (87.4, 87.3, 86.9 and 86.9 % sequence similarity), respectively. The major cellular fatty acids (> 10 %) of the isolate were identified as C-18:1 cis 9 FAME (36.9 %), C-16:0 FAME (33.7 %) and C-18:0 FAME (13.2 %). In contrast to the tested reference strains, C-20:0 FAME (4.0 %) was detected only in strain ALO17(T) whilst C-16:0 DMA was absent. The isolate also differed in its substrate oxidation profiles from the reference strains by being positive for d-melibiose and stachyose but negative for N-acetyl-d-galactosamine and 3-methyl-d-glucose. On the basis of polyphasic taxonomic evidence from this study, the isolate is concluded to belong to a novel genus within the family Erysipelothricaceae. We propose the name Faecalibaculum rodentium gen. nov., sp. nov. to accommodate strain ALO17(T) (=KCTC 15484(T) = JCM 30274(T)) as the type strain.