Proteomic analysis of the effect of heat stress on hexaploid wheat grain:: Characterization of heat-responsive proteins from total endosperm
Proteomic analysis of the effect of heat stress on hexaploid wheat grain:: Characterization of heat-responsive proteins from total endosperm
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DOI：
10.1002/pmic.200390026
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发表时间：
2003-02-01
期刊：
影响因子：
3.4
通讯作者：
Branlard, G
中科院分区：
文献类型：
--
作者：
Majoul, T;Bancel, E;Branlard, G
据报道，灌浆过程中的高温是影响小麦面团特性和品质特性的因素之一。对高温的反应与蛋白质组成在数量和质量水平上的变化有关。利用蛋白质组学方法研究了灌浆过程中高温对面包小麦蛋白质组成的影响。植物在田间种植，开花后不久就被转移到橱柜里。它们受到18℃/10℃(白天/黑夜)和34℃/10℃两种温度制度的影响。从对照籽粒和处理植株三个不同的花后阶段提取总蛋白质。用双向凝胶电泳法分离蛋白质，用Melanie 3软件进行分析。在小麦成熟籽粒蛋白质总量中，有37个蛋白质在热处理过程中发生了显著变化。通过基质辅助激光解吸/电离质谱仪和串联质谱仪的分析，结合数据库搜索，可以鉴定25个热诱导蛋白质和只有一个热还原蛋白质点。为了更多地了解识别出的蛋白质的功能，我们检查了它们在治疗过程中的表达。
High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to determine the influence of high temperature during grain filling on the protein composition of bread wheat evaluated by proteomic tools. Plants were grown in the field and transferred to cabinets soon after flowering. They were subjected to two thermal regimes 18degreesC/10degreesC (day/night) and 34degreesC/10degreesC. Total proteins were extracted from control grains and treated plants at three different post-anthesis stages. The proteins were separated by two-dimensional gel electrophoresis and analysed by Melanie 3 software. Of the total number of mature wheat grain proteins, 37 were identified as significantly changed by heat treatment. Analysis by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry coupled with database searching allowed the characterization of 25 heat-induced proteins and only one heat-decreased protein spot. To learn more about the function of the identified proteins, we examined their expression during treatment.