Investigation on subcellular localization of Rice stripe virus in its vector small brown planthopper by electron microscopy.
Investigation on subcellular localization of Rice stripe virus in its vector small brown planthopper by electron microscopy.
复制标题
电镜研究水稻条纹病毒在小褐飞虱体内的亚细胞定位
DOI：
10.1186/1743-422x-10-310
复制
发表时间：
2013-10-18
期刊：
影响因子：
4.8
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Deng J;Li S;Hong J;Ji Y;Zhou Y
水稻条纹病毒（Rice stripe virus，RSV）是由灰飞虱（Laodelphax striatellus Fallén，SBPH）传播的一种水稻病毒，在包括中国在内的东亚许多国家的稻田中流行并引起严重的水稻条纹叶枯病。研究病毒在载体中的定位对于阐明RSV通过SBPH传播的机制具有重要意义。本研究采用透射电镜和免疫金标记技术，对呼吸道合胞病毒（RSV）核糖核蛋白（RNPs）在小斑潜蝇消化道、肌肉、卵巢和睾丸中的亚细胞定位进行了研究。在带病毒的大豆褐飞虱卵巢管滤泡细胞的胞质基质和液泡中观察到大量电子密度高的无定形RSV包涵体，与水稻细胞中形成的病毒包涵体非常相似。经放大后发现，电子致密包裹体内部形成了砂状或平行的层状结构。大量RSV RNP在蛋壳表面、卵内、中肠腔和上皮细胞中弥散分布，肌肉中病毒含量远低于卵巢和中肠组织。除呼吸道合胞病毒外，虫体内还存在大量的内源性微生物，包括酵母样内共生菌（YLES）、内共生细菌和昆虫病毒。根据病毒定位结果，提出了RSV跨卵传播的可能机制：RSV可能首先在卵泡细胞内含物中复制积累，然后利用营养物质运输途径，通过卵壳，随营养物质沿着传播到卵母细胞中。此外，RSV可能利用肌肉在载体中传播，效率较低。
Rice stripe virus (RSV), which is transmitted by small brown planthopper (Laodelphax striatellus Fallén, SBPH), has been reported to be epidemic and cause severe rice stripe disease in rice fields in many East Asian countries, including China. Investigation on viral localization in the vector is very important for elucidating transmission mechanisms of RSV by SBPH. In this study, transmission electron microscopy and immuno-gold labeling technique were used to investigate the subcellular localization of the ribonucleoproteins (RNPs) of RSV in the digestive tract, muscles, ovary and testes of SBPH. A lot of amorphous RSV inclusion bodies with high electron density were observed in the cytoplasmic matrix and vacuoles of follicular cells of ovarioles in viruliferous SBPH, which were very similar to viral inclusions formed in rice cells. After magnified, it was found that sand-like or parallel filamentary structures were constructed inside the electron-dense inclusions. A large numbers of RSV RNPs distributed diffusely throughout the eggshell surface and interior of ovum, midgut lumen and epithelial cells, while the amount of the virus in muscles was far less than that in the ovary and midgut tissues. Besides RSV, numerous endogenous microorganisms were also observed in SBPH body, including yeast-like endosymbiotes (YLES), endosymbiotic bacteria and insect virus. According to the results of the virus localization, a potential mechanism of RSV transovarial transmission was proposed that RSV might replicate and accumulate initially in the inclusions of follicular cells, then exploit the pathway of the nutrition transportation to pass through the eggshell and spread into the oocytes along with the nutrition. Moreover, RSV might exploit muscles for its spread in vector body with a lower efficiency.