A metabolomic investigation into the temperature-dependent virulence of Pseudomonas plecoglossicida from large yellow croaker (Pseudosciaena crocea)
A metabolomic investigation into the temperature-dependent virulence of Pseudomonas plecoglossicida from large yellow croaker (Pseudosciaena crocea)
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DOI：
10.1111/jfd.12957
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发表时间：
2019-03-01
影响因子：
2.5
通讯作者：
Yan, Qingpi
中科院分区：
文献类型：
--
作者：
Huang, Lixing;Zuo, Yanfei;Yan, Qingpi
绒舌假单胞菌与多种鱼类疾病有关，温度是影响其爆发的最重要环境因素之一。为了阐明温度变化对病原菌的影响，我们分析了P. pleclossicida （NZBD9）在毒力（18℃）和无毒（12℃和28℃）温度下的全球代谢组学。结果表明，磷酸、酪氨酸、亚精胺和蔗糖水平显著降低。衣通酸、葡萄糖酸和异麦芽糖在18℃时增加，这些代谢调节有助于衣通酸在不利环境中存活，在宿主中增殖，在感染的初始阶段定植并抵抗宿主免疫清除。结果表明，L321_03626和L321_18122基因在这些代谢适应的调控中发挥了关键作用，从而调控了pleclossicida在毒温下的毒力，进一步的基因沉默和人工感染证实了这一点。本研究首次确定了pleclossisidida对温度变化的代谢组反应，有助于探索其致病机制，为疾病防治提供参考。
Pseudomonas plecoglossicida is associated with multiple fish diseases, and temperature is one of the most important environmental factors related to its outbreak. To elucidate the influence of temperature variation on the pathogen, the global metabolomics of P. plecoglossicida (NZBD9) were analysed at the virulent (18 degrees C) and avirulent (12 degrees C and 28 degrees C) temperatures. The result showed that the levels of Phosphoric acid, Tyrosine, Spermidine and Sucrose were significantly reduced?while Itaconic acid, Glucaric acid and Isomaltose were increased in P. plecoglossicida at 18 degrees C. These metabolic adjustments assist P. plecoglossicida to survive in adverse environments, proliferate in the host, colonize and resist host immune clearance during the initial steps of infection. The results suggested that L321_03626 and L321_18122 genes played a key role in the regulation of these metabolic adaptions and thus regulated P. plecoglossicida virulence at virulent temperature, which was proved by further gene silencing and artificial infection. The present study, for the first time, determines the P. plecoglossicida metabolomic responses to temperature variation, which is helpful to explore its pathogenic mechanism and provides reference for disease control.