Preservation of DNA
Preservation of DNA
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DOI：
10.1089/cpt.2007.0511
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发表时间：
2007-12-01
影响因子：
--
通讯作者：
Molina, Marion C.
中科院分区：
文献类型：
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作者：
Anchordoquy, Thomas J.;Molina, Marion C.
从生物信息库管理到制药科学，DNA的保存引起了不同领域的科学家的广泛兴趣。尽管“保存”一词被所有这些领域使用，指的是保持DNA分子的化学和物理完整性，但这些不同领域的科学家的观点截然不同也就不足为奇了。最值得注意的是，一种药品的稳定性的时间框架大约是两年，而对于进化生物学家来说，有意义的稳定性是以数亿年来衡量的。这种不同的观点不仅对保存的时间跨度有重大影响，而且对用什么标准来评估“稳定性”也有重大影响。本综述从改进保存方法的角度讨论了有关DNA完整性维护的文献。具体地说，讨论了DNA在溶液、冷冻和干燥时的稳定性。对这些研究的结果进行了比较，并估计了通过当代方法(即冷藏)维护DNA样本的相关成本。考虑到将样品保持在冷冻状态的巨大成本，我们得出结论，在常温下干燥储存对于许多应用来说是足够的，我们建议根据文献中的发现来研究保存策略。此外，我们还提供了一些关于关键研究的建议，这些研究可以用来比较不同领域发表的结果，使用非常不同的标准来评估“稳定性”。
The preservation of DNA is of wide interest to scientists in disparate fields from biorepository management to pharmaceutical sciences. Although the term "preservation" is used by all these fields and refers to the maintenance of chemical and physical integrity of the DNA molecule, it should not be surprising that the perspectives of scientists from these distinct fields differ significantly. Most notably, the time frame for stability of a pharmaceutical product is approximately 2 years, whereas meaningful stability for an evolutionary biologist is measured in the hundreds of millions of years. Such divergent viewpoints not only have a significant effect on the time span of preservation, but also on what criteria are used to assess "stability." This review discusses the literature addressing the maintenance of DNA integrity from the perspective of developing methods that offer improved preservation. Specifically, studies on the stability of DNA in solution, frozen, and dried are discussed. The findings from these studies are compared, and the costs associated with maintaining DNA samples via contemporary methods (i.e., cold storage) are estimated. In light of the significant cost of maintaining samples in the frozen state, we conclude that dry storage at ambient temperatures would be adequate for many applications, and we suggest preservation strategies that should be investigated based on findings in the literature. In addition, we offer suggestions regarding critical studies that could be performed to compare published results from different fields using vastly different criteria to assess "stability.".