Butanol production from alkaline pretreated bamboo using co-culture of wood-rotting fungus Phlebia sp. MG-60-P2 and bacterium Clostridium saccharoperbutylacetonicum
Butanol production from alkaline pretreated bamboo using co-culture of wood-rotting fungus Phlebia sp. MG-60-P2 and bacterium Clostridium saccharoperbutylacetonicum
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DOI：
10.1007/s13399-023-04320-8
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发表时间：
2023-05
影响因子：
4
通讯作者：
C. Tri;L. D. Khuong;Ichiro Kamei
中科院分区：
文献类型：
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作者：
C. Tri;L. D. Khuong;Ichiro Kamei
目前，生物燃料生产的重点是利用木质纤维素生物质中的多糖。本研究以氢氧化钠预处理后的竹材为原料，通过与木腐真菌Phlebiasp共培养的方法生产丁醇。MG-60-P2和细菌C.糖多丁基丙酮细菌单培养不能利用预处理后的竹材，但与P2共培养成功。用5.0%NaOH预处理20.0g/L的毛竹，共培养产生丁醇0.5g/L，丁酸1.3g/L，乙醇0.3g/L。共培养不仅可以得到生物燃料产品，而且可以提高预处理后竹材中多糖的总糖化率。1.0%NaOH预处理与共培养相结合，成功地实现了从多糖到发酵产物的碳（摩尔）的最高理论转化率（46.1%）。这是关于白腐真菌Phlebiasp协同作用的首次报道。MG-60-P2和厌氧细菌C.在一个实施方案中，在共培养物中使用糖多丁基丙酮酸以代谢氢氧化钠预处理的木质纤维素中的纤维素和半纤维素以产生丁醇。
Biofuel production is currently focused on the utilization of polysaccharides in lignocellulosic biomass. In this research, butanol was produced from sodium hydroxide-pretreated bamboo by a co-culture with the wood-rotting fungusPhlebiasp. MG-60-P2 and bacteriumC. saccharoperbutylacetonicum. The bacterial monoculture could not utilize the pretreated bamboo, but the co-culture with P2 was successful. Co-culturing produced 0.5 g/L butanol, 1.3 g/L butyric acid, and 0.3 g/L ethanol from 20.0 g/L bamboo pretreated with 5.0% NaOH. The co-culture not only gave the biofuel products but also enhanced the total saccharification rate of polysaccharides in the pretreated bamboo. A combination of 1.0% NaOH pretreatment with the co-culture succeeded in the highest theoretical conversion (46.1%) of carbon (in moles) from the polysaccharides to the fermentation products. This is the first report on the synergistic effects of white-rot fungusPhlebiasp. MG-60-P2 and anaerobic bacteriumC. saccharoperbutylacetonicumin a co-culture to metabolize cellulose and hemicellulose in sodium hydroxide-pretreated lignocellulose to produce butanol.