Measurement of motility of Helicobacter pylori, Campylobacter jejuni and Escherichia coli by real time computer tracking using the Hobson BacTracker
Measurement of motility of Helicobacter pylori, Campylobacter jejuni and Escherichia coli by real time computer tracking using the Hobson BacTracker
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DOI：
10.1136/jcp.51.8.623
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发表时间：
1998-08-01
影响因子：
3.4
通讯作者：
Worku, ML
中科院分区：
文献类型：
--
作者：
Karim, QN;Logan, RPH;Worku, ML
目的：（1）对幽门螺杆菌、空肠弯曲菌和大肠杆菌这三种胃肠道致病菌在对数生长期的运动特性进行精确的测量和比较;（2）提供运动数据的背景信息，以研究pH、粘度和趋化因子的影响，方法计算机图像处理技术和相差显微镜技术（霍布森BacTracker）对细菌运动性进行了真实的实时测定。10个临床分离的每种在对数期液体culture.Results-C空肠移动最快，与中位曲线速度（CLV）的38.76 μ m/s（范围29.08至52.82）。其次是幽门螺杆菌，中位CLV为25.02 μ m/s（范围12.07至29.07）。大肠杆菌是最慢的，中位CLV 12.73 μ m/s（范围8.20至18.04）。直线速度也有类似的趋势。轨迹线性（TL）的测量结果表明，空肠弯曲菌移动最直（TL 60.3%），幽门螺杆菌移动在宽的圆圈（TL 28.7%），和大肠杆菌显示旋转运动没有太多的线性位移（TL 18.3%）。有这三个变量之间的物种研究的显着差异，但没有显着差异，在测量的时间和频率之间的运动runn.Conclusions-The BacTracker的停顿提供了一个有用的技术援助，用于测量几个指标的细菌运动的客观，重现性，和精确，这是很难实现没有计算机辅助。准确定量细菌运动性为研究影响细菌运动性的因素提供了基础。它可以提供鞭毛基因缺失的影响的表型测量。
Aims-(1) To make precise measurements and comparisons of various aspects of motility of three gastrointestinal pathogens, Helicobacter pylori, Campylobacter jejuni, and Escherichia coli, in log phase growth; (2) to provide background information on motility data to study the influence of pH, viscosity, and chemotactic factors, thereby gaining a better understanding of bacterial pathogenesis.Methods-Computer image processing technology and phase contrast microscopy (Hobson BacTracker) were used to measure several indices of bacterial motility in real time. Ten clinical isolates of each species in log phase Liquid culture were studied.Results-C jejuni moved fastest, with a median curvilinear velocity (CLV) of 38.76 mu m/s (range 29.08 to 52.82). Next was H pylori, median CLV 25.02 mu m/s (range 12.07 to 29.07). E coli was the slowest, median CLV 12.73 mu m/s (range 8.20 to 18.04). The straight line velocities showed similar trends. Measurement of track linearity (TL) showed that C jejuni moved the straightest (TL 60.3%), H pylori moved in wide circles (TL 28.7%), and E coli showed spinning movement without much linear displacement (TL 18.3%). There were significant differences in these three variables between the species studied, but no significant differences in measurements of time and frequency of halts between movement runs.Conclusions-The BacTracker provides a useful technical aid for measuring several indices of bacterial motility objectively, reproducibly, and precisely, which is difficult to achieve without computer assistance. Accurate quantification of motility provides a basis for studying the factors which influence bacterial motility. It can provide phenotypic measurements of the effect of flagellar gene depletion.