Metal-free DNA sensor based on 10-phenylphenothiazine photo-ATRP signal amplification
Metal-free DNA sensor based on 10-phenylphenothiazine photo-ATRP signal amplification
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DOI：
10.1016/j.microc.2023.108816
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发表时间：
2023-05
影响因子：
4.8
通讯作者：
Shuaibing Yu;Lianzhi Li;J. Kong;Xueji Zhang
中科院分区：
文献类型：
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作者：
Shuaibing Yu;Lianzhi Li;J. Kong;Xueji Zhang
与该病相关的超灵敏脱氧核糖核酸（DNA）传感器的开发对于该病的早期诊断和预防具有重要意义。本文采用光催化原子转移自由基聚合（photo-ATRP）技术制备了一种超灵敏的DNA传感器。该策略利用巯基修饰的肽核酸（PNA）特异性识别靶DNA，并通过磷酸锆（IV）-羧酸盐将α-溴苯乙酸（BPAA）接枝到DNA上。在光催化剂10-苯基吩噻嗪（PTH）存在下，α-Br在365 nm紫外光下被还原产生活性自由基，通过介导ATRP将含电化学信号分子（二茂铁）的聚合物链引入电极表面。最后，通过方波伏安法（SWV）测量峰电流值，并评估DNA的浓度。该传感器基于ATRP反应的绿色策略构建，不使用重金属催化剂（无金属），从而减少了对环境的污染。在最佳条件下，传感器与目标浓度在0.1 fM ~ 10 pM范围内呈线性关系，检测限为79 aM。该传感器具有灵敏度高、抗干扰、稳定性好等优点，在生命科学和医学研究领域具有很大的实际应用潜力。
The development of ultrasensitive deoxyribonucleic acid (DNA) sensors related to the disease is of great significance for early diagnosis and prevention of the disease. Here, an ultrasensitive DNA sensor based on the photocatalytic atom transfer radical polymerization (photo-ATRP) strategy was prepared. In this strategy, the target DNA was specifically recognized by sulfhydryl-modified peptide nucleic acid (PNA), and α-bromophenylacetic acid (BPAA) was grafted onto the DNA via phosphate-Zr(IV)-carboxylate. In the presence of photocatalyst 10-phenylphenothiazine (PTH), α-Br was reduced under 365 nm UV light to generate active radicals, and the polymer chain containing electrochemical signaling molecule (ferrocene) was introduced to the electrode surface by mediated ATRP. Finally, the peak current value was measured by square wave voltammetry (SWV) and the concentration of DNA was evaluated. The biosensor was constructed based on a green strategy for ATRP reaction without using a heavy metal catalyst (metal-free), thus reducing the pollution of the environment. Moreover, under optimal conditions, the relationship between the DNA sensor and the target was linear in the concentration range of 0.1 fM ∼ 10 pM, and the limit of detection was 79 aM. The sensor has the advantages of high sensitivity, interference-free and good stability, which indicates that it has great practical application potential in the field of life science and medical research.