Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells
Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells
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DOI：
10.1007/7651_2015_234
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Dalton, Stephen
中科院分区：
文献类型：
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作者：
Avery, John;Dalton, Stephen
多能神经嵴细胞（NCC）在胚胎发育过程中产生多种细胞类型。这包括黑素细胞、外周神经元、平滑肌细胞、骨细胞、软骨细胞和脂肪细胞。这里描述的方案允许在15天内高效地将人多能干细胞分化为神经嵴命运。这是在无饲养层条件下使用补充有两种小分子抑制剂的化学成分确定的培养基完成的，所述小分子抑制剂阻断糖原合成酶激酶3（GSK 3）和骨形态发生蛋白（BMP）信号传导。这项技术非常适合作为一个平台，以更详细地了解与受损的神经嵴发育/迁移相关的人类疾病的发病机制。
Multipotent, neural crest cells (NCCs) produce a wide range of cell types during embryonic development. This includes melanocytes, peripheral neurons, smooth muscle cells, osteocytes, chondrocytes, and adipocytes. The protocol described here allows for highly efficient differentiation of human pluripotent stem cells to a neural crest fate within 15 days. This is accomplished under feeder-free conditions, using chemically defined medium supplemented with two small molecule inhibitors that block glycogen synthase kinase 3 (GSK3) and bone morphogenic protein (BMP) signaling. This technology is well suited as a platform to understand in greater detail the pathogenesis of human disease associated with impaired neural crest development/migration.