CYCLIC 3,5-NUCLEOTIDE PHOSPHODIESTERASE . DEMONSTRATION OF AN ACTIVATOR
CYCLIC 3,5-NUCLEOTIDE PHOSPHODIESTERASE . DEMONSTRATION OF AN ACTIVATOR
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DOI：
10.1016/0006-291x(70)90747-3
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发表时间：
1970-01-01
影响因子：
3.1
通讯作者：
CHEUNG, WY
中科院分区：
文献类型：
--
作者：
CHEUNG, WY
纯化的环状3′，5 ′-核苷酸磷酸二酯酶和粗品的混合物的活性大于单独制剂的活性总和。粗酶含有活化剂，其在纯化过程中从纯化的酶中除去。分离的无磷酸二酯酶活性的激活剂有效地重建了纯化酶的活性。纯化的磷酸二酯酶的相对无活性是由于从酶中去除了激活剂。
The activity of a mixture of a purified and a crude cyclic 3′,5′-nucleotide phosphodiesterase was greater than the summed activities of the individual preparations. The crude enzyme contained an activator, which was removed from the purified enzyme during purification. The activator, isolated free of phosphodiesterase activity, effectively reconstituted the activity of the purified enzyme. The relative inactivity of purified phosphodiesterase was due to removal of the activator from the enzyme.