Complete Mitochondrial Genome of the Red Fox (Vuples vuples) and Phylogenetic Analysis with Other Canid Species.
Complete Mitochondrial Genome of the Red Fox (Vuples vuples) and Phylogenetic Analysis with Other Canid Species.
复制标题
DOI：
10.3724/sp.j.1141.2010.02122
复制
发表时间：
2010-04
期刊：
影响因子：
--
通讯作者：
H. Zhong;Hong-hai Zhang;Weilai Sha;C. Zhang;Yu-Cai Chen
中科院分区：
文献类型：
--
作者：
H. Zhong;Hong-hai Zhang;Weilai Sha;C. Zhang;Yu-Cai Chen
测定了赤狐（Vuples vuples）线粒体基因组全序列。该基因全长16 723 bp。与大多数哺乳动物线粒体基因组一样，它包含13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因和1个控制区。基础成分分别为31.3%A、26.1%C、14.8%G和27.8%T。赤狐、北极狐、灰狼、家犬和郊狼的密码子使用遵循相同的模式，除了一个不寻常的ATT起始密码子，该起始密码子启动赤狐中的NADH脱氢酶亚基3基因。在控制区的保守序列块1和2之间发现了富含AC的长串联重复序列。为了确定赤狐与其它犬科动物的系统发育关系，采用邻接法和最大简约法构建赤狐与其它犬科动物的系统发育树。结果表明，北极狐是赤狐的姐妹群，它们都属于犬科的赤狐类分支，而灰狼、家犬和郊狼属于狐类分支。该结果与已有的系统发育结果一致。
The whole mitochondrial genome sequence of red fox (Vuples vuples) was determined. It had a total length of 16 723 bp. As in most mammal mitochondrial genome, it contained 13 protein coding genes, two ribosome RNA genes, 22 transfer RNA genes and one control region. The base composition was 31.3% A, 26.1% C, 14.8% G and 27.8% T, respectively. The codon usage of red fox, arctic fox, gray wolf, domestic dog and coyote followed the same pattern except for an unusual ATT start codon, which initiates the NADH dehydrogenase subunit 3 gene in the red fox. A long tandem repeat rich in AC was found between conserved sequence block 1 and 2 in the control region. In order to confirm the phylogenetic relationships of red fox to other canids, phylogenetic trees were reconstructed by neighbor-joining and maximum parsimony methods using 12 concatenated heavy-strand protein-coding genes. The result indicated that arctic fox was the sister group of red fox and they both belong to the red fox-like clade in family Canidae, while gray wolf, domestic dog and coyote belong to wolf-like clade. The result was in accordance with existing phylogenetic results.