Micropatterning Facilitates the Long-Term Growth and Analysis of iPSC-Derived Individual Human Neurons and Neuronal Networks.
Micropatterning Facilitates the Long-Term Growth and Analysis of iPSC-Derived Individual Human Neurons and Neuronal Networks.
复制标题
微图案促进了IPSC衍生的人类神经元和神经元网络的长期增长和分析。
DOI：
10.1002/adhm.201500900
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发表时间：
2016-08
影响因子：
10
通讯作者：
Krainc D
中科院分区：
文献类型：
--
作者：
Burbulla LF;Beaumont KG;Mrksich M;Krainc D
The discovery of induced pluripotent stem cells (iPSC) and their application to patient-specific disease models offers new opportunities for studying the pathophysiology of neurological disorders. However, current methods for culturing iPSC-derived neuronal cells result in clustering of neurons, which precludes the analysis of individual neurons and defined neuronal networks. To address this challenge, we developed cultures of human neurons on micropatterned surfaces that promote neuronal survival over extended periods of time. This approach facilitates studies of neuronal development, cellular trafficking, and related mechanisms that require assessment of individual neurons and specific network connections. Importantly, micropatterns support the long-term stability of cultured neurons, which enables time-dependent analysis of cellular processes in living neurons. The approach described in this paper will allow mechanistic studies of human neurons, both in terms of normal neuronal development and function, as well as time-dependent pathological processes, and will provide a platform for testing of new therapeutics in neuropsychiatric disorders.
