Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells.
Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells.
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DOI：
10.1016/j.euprot.2017.06.001
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发表时间：
2017-09
影响因子：
--
通讯作者：
Junqueira M
中科院分区：
文献类型：
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作者：
Murillo JR;Goto-Silva L;Sánchez A;Nogueira FCS;Domont GB;Junqueira M
采用iTRAQ策略对SH-SY5Y细胞进行分化分析。分化后的SH-SY5Y细胞更适合作为神经元模型。上调蛋白主要与ecm相互作用和细胞凋亡有关。作为分化标记的蛋白：AGRN， EMILIM-1， AIFM, STMN1。SH-SY5Y神经母细胞瘤细胞在维甲酸（RA）和脑源性神经营养因子（BDNF）的作用下易分化，提供了神经元分化的模型。我们使用iTRAQ和磷酸肽富集策略比较了RA/BDNF处理前后SH-SY5Y细胞的蛋白质组。我们鉴定了5587个蛋白质，其中366个具有差异丰度。分化细胞表达与神经元发育相关的蛋白，未分化细胞表达与细胞增殖相关的蛋白。相互作用网络涵盖局灶黏附、细胞骨架动力学和神经退行性疾病过程以及丝裂原活化蛋白激酶相关信号通路的调节；参与这些过程的关键蛋白可能作为神经元分化的标记物被探索。
Differentiation analysis of SH-SY5Y cells with iTRAQ strategy is proposed. Differentiated SH-SY5Y cells are more appropriated as a neuronal model. Upregulated proteins are mainly related to ECM-interaction and apoptosis. Proteins to explore as differentiation markers: AGRN, EMILIM-1, AIFM, STMN1. SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.