Localization of glucuronoxylans in Japanese beech visualized by immunogold labelling
Localization of glucuronoxylans in Japanese beech visualized by immunogold labelling
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DOI：
10.1007/bf01282549
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发表时间：
1998-01-01
期刊：
影响因子：
2.9
通讯作者：
Fujita, M
中科院分区：
文献类型：
--
作者：
Awano, T;Takabe, K;Fujita, M
从小鼠体内制备了一种抗葡萄糖醛酸木聚糖(GXS)的抗血清。斑点免疫分析和竞争抑制试验表明，抗体能与GXS特异性结合。因此，用该抗血清进行免疫金标记法研究GXS在日本山毛榉中的定位。GXS标记仅见于木质部细胞的次生壁，而未见于初生壁和中层。GXs在次生壁中分布均匀，但外次生壁外层GXs含量较少。在细胞壁形成过程中，S-1、S-2和S-3次生壁各层的标记密度均增加。这一结果强烈地表明，GXS的沉积是以穿透性的方式发生的。
An antiserum against glucuronoxylans (GXs) has been raised from a mouse. The dot-blot immunoassay and competitive inhibition test indicated that the antibodies could bind specifically to GXs. Therefore, the antiserum was used for immunogold labelling to investigate the localization of GXs in Japanese beech. Labelling of GXs was seen only in the secondary walls of xylem cells, but not in the primary walls or the middle lamella. GXs were evenly distributed in the secondary walls except for the outer part of the outer secondary-wall layer in which they were less abundant. The labelling density in each secondary-wall layer (S-1, S-2, and S-3) increased during cell wall formation. This result strongly suggests that the deposition of GXs occurs in a penetrative way.